論文 - 詳細
| RRC ID | 39117 |
|---|---|
| 著者 | Okada S, Zhang H, Hatano M, Tokuhisa T. |
| タイトル | A physiologic role of Bcl-xL induced in activated macrophages. |
| ジャーナル | J Immunol |
| Abstract |
Activated macrophages produce nitric oxide (NO) that is an important effector molecule for their antimicrobial and antitumor activities. Since this NO is also toxic for themselves, they have self-defense mechanisms. To elucidate the mechanisms in a physiologic condition, expression of bcl-2 family genes were examined in peritoneal macrophages and RAW264 macrophage cell line activated with IFN-gamma and LPS. Bcl-xL, but not bcl-2 and bax mRNA, was highly inducible within 3 h after stimulation. The induction required new protein synthesis, but was independent of effects of synthesized NO. Since activated RAW264 were more resistant to NO-induced apoptosis mediated by the exposure to S-nitroso-N-acetyl-penicillamine (SNAP) than nonactivated RAW264, the inducible Bcl-xL may play a role in the protection from NO toxicity. To confirm the protective function, RAW264 were stably transfected with bcl-xL. Those transfectants activated with IFN-gamma and LPS appeared highly resistant to NO-induced cell death detected within 24 h after stimulation, although their NO production was similar to those of parental RAW264 and neomycin control-transfected cells. Furthermore, bcl-xL transfectants displayed substantial protection from SNAP-induced apoptosis. These results establish a link between self-defense to the synthesized NO and the induction of Bcl-xL in activated macrophages. |
| 巻・号 | 160(6) |
| ページ | 2590-6 |
| 公開日 | 1998-3-15 |
| PMID | 9510156 |
| MeSH | Animals Cell Death Cell Line Interferon-gamma / pharmacology Lipopolysaccharides / pharmacology Macrophage Activation Macrophages / physiology* Mice Mice, Inbred C57BL Nitric Oxide / physiology Proto-Oncogene Proteins c-bcl-2 / genetics Proto-Oncogene Proteins c-bcl-2 / physiology* RNA, Messenger / analysis bcl-X Protein |
| IF | 4.886 |
| 引用数 | 64 |
| WOS 分野 | IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |