| RRC ID |
39146
|
| 著者 |
Endo K, Anada T, Yamada M, Seki M, Sasaki K, Suzuki O.
|
| タイトル |
Enhancement of osteoblastic differentiation in alginate gel beads with bioactive octacalcium phosphate particles.
|
| ジャーナル |
Biomed Mater
|
| Abstract |
The present study investigated whether alginate (Alg) hydrogel microbeads have a role in maintaining mouse bone marrow stromal ST-2 cells and release the cells after being stimulated by synthetic octacalcium phosphate (OCP), which is a mineral crystal capable of stimulating osteoblastic differentiation during a conversion process to hydroxyapatite (HA). The ST-2 cell suspension in the alginate solution, which contained various concentrations of OCP granules with diameters less than 53 μm, was extruded drop-wise into a stirred gelation solution containing BaCl2 using an encapsulator with nitrogen gas stream. The Alg-microbeads (Alg/OCP · ST-2 microbeads) that were generated, which had a diameter of approximately 400 μm, were incubated for up to 14 d and then assessed for osteoblastic differentiation. Alg-microbeads with cells were also incubated to identify the possible conversion from OCP to HA. Osteoblast differentiation markers in ST-2 cells, alkaline phosphatase (ALP) and collagen type I, were up-regulated in the presence of higher amounts of OCP. X-ray diffraction analysis and Fourier transform infrared spectroscopy confirmed that the OCP tended to convert to HA over time, suggesting that the OCP in Alg-microbeads interacts three-dimensionally with ST-2 cells and stimulates its osteoblastic differentiation. The release of ST-2 cells from the microbeads was also estimated. ST-2 cells were identified outside of the microbeads, although the cell number tended to decrease with increasing OCP. These results suggest that Alg/OCP microbeads could be used as a vehicle to activate osteoblastic cells and deliver them to sites where bone regeneration is needed.
|
| 巻・号 |
10(6)
|
| ページ |
065019
|
| 公開日 |
2015-12-14
|
| DOI |
10.1088/1748-6041/10/6/065019
|
| PMID |
26657659
|
| MeSH |
Alginates / chemistry*
Animals
Bone Substitutes / chemical synthesis
Calcium Phosphates / chemistry*
Cell Differentiation / physiology
Cell Line
Gels / chemistry
Glucuronic Acid / chemistry
Hexuronic Acids / chemistry
Mesenchymal Stem Cells / cytology*
Mesenchymal Stem Cells / physiology*
Mice
Microspheres
Osteoblasts / cytology*
Osteoblasts / physiology*
Osteogenesis / physiology
Tissue Scaffolds
|
| IF |
3.174
|
| 引用数 |
4
|
|
WOS 分野
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
| リソース情報 |
| ヒト・動物細胞 |
ST2(RCB0224) |
