RRC ID 39187
Author Taketsuru H, Kaneko T.
Title In vitro maturation of immature rat oocytes under simple culture conditions and subsequent developmental ability.
Journal J Reprod Dev
Abstract Rat oocytes can be produced artificially by superovulation. Because some strains show low sensitivity to superovulation treatment, in vitro maturation is an alternative method to produce numerous matured oocytes. Furthermore, establishment of an in vitro maturation system with simple culture conditions is cost effective and leads to easy handling of oocytes. This study examined developmental ability of rat germinal vesicle (GV) oocytes maturing in vitro under simple culture conditions. Significantly different numbers of ovulated oocytes reached the second metaphase of meiosis (MII) among Jcl:Wistar (17.0), F344/Stm (31.0), and BN/SsNSlc (2.2) rats in whom superovulation was induced by pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin. However, similar numbers of GV oocytes were obtained from ovaries of PMSG-injected Wistar (27.7), F344 (34.7), and BN (24.7) rats. These GV oocytes were cultured in vitro in HTF, αMEM, and a 1:1 HTF + αMEM or TYH + αMEM mixture. High proportions of Wistar and F344 oocytes that matured to MII in αMEM were parthenogenetically activated by strontium chloride treatment (78% and 74%, respectively). Additionally, 10% of matured oocytes of both strains developed into offspring after intracytoplasmic sperm injection and embryo transfer to foster mothers. Although BN oocytes cultured in αMEM could be parthenogenetically activated and developed into offspring, the success rate was lower than that for Wistar and F344 oocytes. This study demonstrated that numerous GV oocytes were produced in rat ovaries by PMSG injection. This simple in vitro maturation system of immature oocytes could be further developed to maintain valuable rat strains experiencing reproductive difficulties.
Volume 62(5)
Pages 521-526
Published 2016-10-18
DOI 10.1262/jrd.2016-057
PMID 27375268
PMC PMC5081740
MeSH Animals Cell Culture Techniques* Embryo Transfer Female Fertilization in Vitro Gonadotropins, Equine / metabolism Male Oocytes / cytology* Ovary / metabolism Parthenogenesis / drug effects Rats Rats, Inbred BN Rats, Inbred F344 Rats, Wistar Sperm Injections, Intracytoplasmic
IF 1.735
Times Cited 1
Rats F344/Stm(strainID=266)