Reference - Detail
| RRC ID | 39188 |
|---|---|
| Author | Ramos MA, Kuzuya M, Esaki T, Miura S, Satake S, Asai T, Kanda S, Hayashi T, Iguchi A. |
| Title | Induction of macrophage VEGF in response to oxidized LDL and VEGF accumulation in human atherosclerotic lesions. |
| Journal | Arterioscler Thromb Vasc Biol |
| Abstract |
The interaction between macrophages and oxidatively modified low density lipoprotein (Ox-LDL) appears to play a central role in the development of atherosclerosis, not only through foam cell formation but also via the induction of numerous cytokines and growth factors. The current study demonstrated that Ox-LDL upregulated vascular endothelial growth factor (VEGF) mRNA expression in RAW 264 cells, a monocytic cell line, in a time- and concentration-dependent manner and that Ox-LDL stimulated VEGF protein secretion from the cells. Lysophosphatidylcholine, a component of Ox-LDL, also enhanced VEGF mRNA expression in RAW 264 cells and VEGF secretion from RAW 264 cells, with a maximal effect at a concentration of 10 micromol/L lysophosphatidylcholine. Immunohistochemical studies showed that human early atherosclerotic lesions exhibited intense VEGF immunoreactivity in subendothelial macrophage-rich regions of the thickened intima. In atherosclerotic plaques, VEGF staining was also observed in foam cell-rich regions adjacent to the lipid core or the neovascularized basal regions of plaque consisting predominantly of smooth muscle cells. High-power-field observation revealed that VEGF was localized in the extracellular space as well as at the macrophage cell surface. These observations suggest the possible involvement of Ox-LDL in the development of human atherosclerosis through VEGF induction in macrophages. |
| Volume | 18(7) |
| Pages | 1188-96 |
| Published | 1998-7-1 |
| DOI | 10.1161/01.atv.18.7.1188 |
| PMID | 9672081 |
| MeSH | 3T3 Cells Animals Arteriosclerosis / metabolism* Blotting, Northern Culture Media, Conditioned Endothelial Growth Factors / analysis Endothelial Growth Factors / biosynthesis* Endothelial Growth Factors / genetics Gene Expression Regulation / drug effects Humans Immunohistochemistry Lipoproteins, LDL / pharmacology* Lymphokines / analysis Lymphokines / biosynthesis* Lymphokines / genetics Lysophosphatidylcholines / pharmacology Macrophages / metabolism* Mice RNA, Messenger / metabolism Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factors |
| IF | 6.604 |
| Times Cited | 122 |
| WOS Category | HEMATOLOGY PERIPHERAL VASCULAR DISEASE |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | RAW 264(RCB0535) |