論文 - 詳細
| RRC ID | 39192 |
|---|---|
| 著者 | Murakami Y, Nobukuni T, Tamura K, Maruyama T, Sekiya T, Arai Y, Gomyou H, Tanigami A, Ohki M, Cabin D, Frischmeyer P, Hunt P, Reeves RH. |
| タイトル | Localization of tumor suppressor activity important in nonsmall cell lung carcinoma on chromosome 11q. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
Loss of heterozygosity on chromosome 11q23 is observed at high frequency in human nonsmall cell lung carcinomas (NSCLCs), suggesting the presence of a tumor suppressor gene. Previous analysis of DNA from 79 patients identified a commonly deleted segment of 5 centimorgans. Complementation analysis was used to further localize a putative tumor suppressor gene. Three yeast artificial chromosome (YAC) clones spanning the minimal loss of heterozygosity region were modified, and spheroplast fusion was used to transfer them into human A549 NSCLC or murine Lewis lung carcinoma (LLC) cell lines. The resulting yeast x human hybrid cell lines containing an intact copy of a 1.6-Mb YAC, 939b12, showed reduced growth in vitro. Injection of parental A549 cells into athymic (nu/nu) mice resulted in tumor formation at 27 of 28 injection sites. In contrast, two independent 939b12-containing cell lines formed tumors at only 3 of 20 injection sites. 939b12 also suppressed tumor formation by LLC NSCLC cells in nude mice, but YACs 785e12 and 911f2, which flank 939b12, had no suppressor activity. Further localization of tumor suppression activity on 939b12 was accomplished by introduction of defined fragmentation derivatives into A549 cells and by analysis of YACs that were broken on transfer into LLC cells. This complementation approach localized tumor suppression activity to the central 700 kb of 939b12 and provides a functional assay for positional cloning of this tumor suppressor gene. |
| 巻・号 | 95(14) |
| ページ | 8153-8 |
| 公開日 | 1998-7-7 |
| DOI | 10.1073/pnas.95.14.8153 |
| PMID | 9653156 |
| PMC | PMC20945 |
| MeSH | Animals Carcinoma, Non-Small-Cell Lung / genetics* Chromosome Mapping Chromosomes, Artificial, Yeast Chromosomes, Human, Pair 11* Genes, Tumor Suppressor* Humans Lung Neoplasms / genetics* Mice Mice, Nude Neoplasms, Experimental / genetics Neoplasms, Experimental / pathology |
| IF | 9.412 |
| 引用数 | 74 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | A549(RCB0098) LLC(RCB0558) |