Reference - Detail
| RRC ID | 39207 |
|---|---|
| Author | Komatsu N, Oda T, Muramatsu T. |
| Title | Involvement of both caspase-like proteases and serine proteases in apoptotic cell death induced by ricin, modeccin, diphtheria toxin, and pseudomonas toxin. |
| Journal | J Biochem |
| Abstract |
We investigated the involvement of caspases and serine proteases in apoptotic cell death induced by ricin, modeccin, diphtheria toxin, and Pseudomonas toxin in U937 cells. We found that caspase-3- and caspase-6-like activities, but not caspase-1-like activity, increased during toxin-induced apoptosis. Z-D-CH2-DCB, a caspase-like inhibitor, completely inhibited the generation of caspase-3- and caspase-6-like activities and blocked all features of apoptosis induced by toxins: nuclear morphological changes, DNA fragmentation, and cytotoxicity. However, three caspase-specific inhibitors, Ac-YVAD-CHO, Ac-DEVD-CHO, and Ac-VEID-CHO, had no effect, even though Ac-DEVD-CHO and Ac-VEID-CHO inhibited the increased caspase-3- and caspase-6-like activity, respectively. These results suggest that the generation of caspase-3- and caspase-6-like activities is redundant, and other caspases distinct from caspase-3 and -6 may be important in toxin-induced apoptosis. Furthermore, serine protease inhibitor, 3,4-dichloroisocoumarine (DCI), abolished the apoptotic cell death and DNA fragmentation caused by toxins, without affecting the increased caspase-3- and caspase-6-like activities. Our results suggest that multiple proteases with different preferences for apoptotic substrates participate in toxin-induced apoptotic death of U937 cells. |
| Volume | 124(5) |
| Pages | 1038-44 |
| Published | 1998-11-1 |
| DOI | 10.1093/oxfordjournals.jbchem.a022197 |
| PMID | 9792931 |
| MeSH | ADP Ribose Transferases* Apoptosis / drug effects* Bacterial Toxins* Cell Line Cysteine Endopeptidases / metabolism* Diphtheria Toxin / pharmacology* Exotoxins / pharmacology* Humans Lectins / pharmacology* Plant Lectins* Protein Synthesis Inhibitors / pharmacology Ribosome Inactivating Proteins, Type 2 Ricin / pharmacology* Serine Endopeptidases / metabolism* Virulence Factors* |
| IF | 2.476 |
| Times Cited | 86 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Resource | |
| Human and Animal Cells | U-937 DE-4(RCB0435) |