Reference - Detail
|Author||Ando S, Nojima K, Majima H, Ishihara H, Suzuki M, Furusawa Y, Yamaguchi H, Koike S, Ando K, Yamauchi M, Kuriyama T.|
|Title||Evidence for mRNA expression of vascular endothelial growth factor by X-ray irradiation in a lung squamous carcinoma cell line.|
Vascular endothelial growth factor (VEGF) is a multipotent cytokine which plays an important role in various angiogenic conditions as well as in some tumor behaviors. Here we examined the induction of VEGF mRNA by X-ray irradiation in a lung squamous cell carcinoma cell line (RERF-LC-AI). Irradiating the cells with 15 Gy X-rays significantly increased the mRNA expression up to 2.5-fold of control at a post-irradiation time of 16-24 h. The induction of VEGF mRNA by X-ray irradiation was completely blocked by treating cells with either genistein (Src tyrosine kinase inhibitor) or H7 (protein kinase C inhibitor). This suggests that the mechanism of induction might be concerned with the pathway which triggers Src tyrosine kinase of the cell surface and the protein kinase C pathway.
|MeSH||Antineoplastic Agents / pharmacology Carcinoma, Squamous Cell / genetics Carcinoma, Squamous Cell / pathology Endothelial Growth Factors / genetics* Gene Expression Regulation, Neoplastic / drug effects Gene Expression Regulation, Neoplastic / radiation effects Genistein / pharmacology Humans Lung Neoplasms / genetics Lung Neoplasms / pathology Lymphokines / genetics* RNA, Messenger / genetics* Time Factors Tumor Cells, Cultured / drug effects Tumor Cells, Cultured / metabolism Tumor Cells, Cultured / radiation effects Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factors X-Rays|
|Human and Animal Cells|