論文 - 詳細
| RRC ID | 39215 |
|---|---|
| 著者 | Horie-Sakata K, Shimada T, Hiraishi H, Terano A. |
| タイトル | Role of cyclooxygenase 2 in hepatocyte growth factor-mediated gastric epithelial restitution. |
| ジャーナル | J Clin Gastroenterol |
| Abstract |
Migration of epithelial cells (restitution) is an essential step in the repair of gastric mucosal lesions. Although a variety of growth factors are reported to facilitate gastric epithelial restitution, the intracellular mechanisms of this process are not fully understood. In this study we investigated the effects of hepatocyte growth factor (HGF) on restitution of normal rat gastric epithelial RGM-1 cell monolayers after injury and examined whether cyclooxygenase-2 (COX-2) is involved in HGF-mediated epithelial restitution. Restitution of RGM-1 monolayers was assessed using a round wound restitution model. Application of HGF (5 ng/ml) significantly facilitated the restitution of RGM-1 monolayers after artificial wounding. HGF also induced expression of COX-2 protein in RGM-1 cells, and wounding itself induced COX-2 expression in the cells located at the edge of the wound. Inhibition of COX-2 activity by NS-398, a specific COX-2 inhibitor, significantly delayed the HGF-mediated restitution. These results suggest the involvement of COX-2 in the action of HGF on gastric epithelial restitution. |
| 巻・号 | 27 Suppl 1 |
| ページ | S40-6 |
| 公開日 | 1998-1-1 |
| DOI | 10.1097/00004836-199800001-00008 |
| PMID | 9872497 |
| MeSH | Animals Cell Line Cell Movement / drug effects Cell Movement / physiology* Cyclooxygenase 2 Cyclooxygenase 2 Inhibitors Cyclooxygenase Inhibitors / pharmacology Epithelial Cells / cytology Gastric Mucosa / cytology* Gastric Mucosa / pathology Hepatocyte Growth Factor / metabolism* Immunohistochemistry Isoenzymes / metabolism* Nitrobenzenes / pharmacology Prostaglandin-Endoperoxide Synthases / metabolism* Rats Stomach Ulcer / pathology Stomach Ulcer / physiopathology* Sulfonamides / pharmacology |
| IF | 2.973 |
| 引用数 | 17 |
| WOS 分野 | GASTROENTEROLOGY & HEPATOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | RGM1(RCB0876) |