| RRC ID |
39237
|
| 著者 |
Hara K, Sugimoto C, Kitamura T, Aoki N, Taguchi F, Yogo Y.
|
| タイトル |
Archetype JC virus efficiently replicates in COS-7 cells, simian cells constitutively expressing simian virus 40 T antigen.
|
| ジャーナル |
J Virol
|
| Abstract |
JC polyomavirus (JCV), the causative agent of progressive multifocal leukoencephalopathy (PML), is ubiquitous in humans, infecting children asymptomatically and then persisting in the kidney. Renal JCV is not latent but replicates to excrete progeny in the urine. The renal-urinary JCV DNAs carry the archetype regulatory region that generates various rearranged regulatory regions occurring in JCVs derived from the brains of PML patients. Tissue cultures that support the efficient growth of archetype JCV have not been reported. We studied whether archetype JCV could replicate in COS-7 cells, simian cells transformed with an origin-defective mutant of simian virus 40 (SV40). Efficient JCV replication, as detected by a hemagglutination assay, was observed in cultures transfected with five of the six archetype DNAs. The progeny JCVs could be passaged to fresh COS-7 cells. However, when the parental cells of COS-7 not expressing T antigen were transfected with archetype JCV DNAs, no viral replication was detected, indicating that SV40 T antigen is essential for the growth of JCV in COS-7 cells. The archetype regulatory region was conserved during viral growth in COS-7 cells, although a small proportion of JCV DNAs underwent rearrangements outside the regulatory region. We then attempted to recover archetype JCV from urine by viral culture in COS-7 cells. Efficient JCV production was observed in COS-7 cells infected with five of the six JCV-positive urine samples examined. Thus, COS-7 cells should be of use not only for the production of archetype JCV on a large scale but also for the isolation of archetype JCV from urine.
|
| 巻・号 |
72(7)
|
| ページ |
5335-42
|
| 公開日 |
1998-7-1
|
| DOI |
10.1128/JVI.72.7.5335-5342.1998
|
| PMID |
9620986
|
| PMC |
PMC110153
|
| MeSH |
Aged
Aged, 80 and over
Animals
Antigens, Polyomavirus Transforming / physiology*
COS Cells
DNA, Viral / analysis
DNA, Viral / chemistry
Genes, Regulator
Humans
JC Virus / physiology*
Male
Middle Aged
Simian virus 40 / immunology*
Virus Replication*
|
| IF |
4.501
|
| 引用数 |
37
|
|
WOS 分野
|
VIROLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
COS-7(RCB0539)
CV-1(RCB0160) |
