| RRC ID |
39239
|
| Author |
Sakurai T, Wakimoto N, Yamada M, Shimamura S, Motoyoshi K.
|
| Title |
Effect of macrophage colony-stimulating factor on mouse NK 1.1+ cell activity in vivo.
|
| Journal |
Int J Immunopharmacol
|
| Abstract |
The effect of recombinant human macrophage colony-stimulating factor (rhM-CSF) on NK 1.1+ cell activity in vivo and in vitro was studied. An intravenous injection of rhM-CSF increased the numbers of NK 1.1+ cells in mouse spleen and blood and augmented the clearance of Yac-1 cells in vivo. Using a magnetic cell sorter (MACS), we purified NK 1.1+ cells from vehicle-injected and rhM-CSF-injected mouse spleen cells. More than 95% of the collected cells were NK 1.1 antigen-positive. NK 1.1+ cells purified from rhM-CSF-injected mouse spleen cells exhibited (a) higher cytotoxic activity against Yac-1 cells, (b) higher proliferative responsiveness to interleukin (IL)-2 and (c) a greater production of interferon (IFN)-gamma in response to IL-2 and IL-12 compared to cells purified from vehicle-injected mouse spleen cells in vitro. These results suggest that the administration of rhM-CSF increases NK 1.1+ cell numbers and activates the cells in vivo.
|
| Volume |
20(8)
|
| Pages |
401-13
|
| Published |
1998-8-1
|
| DOI |
10.1016/s0192-0561(98)00040-x
|
| PII |
S0192-0561(98)00040-X
|
| PMID |
9778101
|
| MeSH |
Animals
Cell Count / drug effects
Cytotoxicity, Immunologic
Flow Cytometry
Humans
Interferon-gamma / biosynthesis
Interleukin-2 / pharmacology
Killer Cells, Natural / drug effects*
Killer Cells, Natural / immunology*
Lymphocyte Activation / drug effects
Macrophage Colony-Stimulating Factor / blood
Macrophage Colony-Stimulating Factor / pharmacology*
Male
Mice
Mice, Inbred C57BL
Spleen / cytology
Spleen / immunology
|
| Times Cited |
13
|
|
WOS Category
|
PHARMACOLOGY & PHARMACY
IMMUNOLOGY
|
| Resource |
| Human and Animal Cells |
YAC-1(RCB1165)
P-815(RCB1167)
EL4(RCB1641) |
