| RRC ID |
39241
|
| Author |
Kobayashi T, Sugimoto T, Kanzawa M, Chihara K.
|
| Title |
Identification of an enhancer sequence in 5'-flanking region of 1A exon of mouse liver/bone/kidney-type alkaline phosphatase gene.
|
| Journal |
Biochem Mol Biol Int
|
| Abstract |
The 5'-flanking region of the 1A exon of the mouse liver/bone/kidney-type alkaline phosphatase (L/B/K ALP) gene was analyzed for the promoter activity. Luciferase assays revealed that a segment containing a 9 bp inverted repeat (nt -1212 to -1179, designated as IRS for inverted repeat segment) had an enhancer activity. Though an E-box at nt -234 did not have a prominent promoter activity, the effect of IRS required its presence. Both transcriptional activity and IRS binding to the nuclear proteins were similarly observed even in cells that did not express ALP. Bone morphogenetic protein-2 (BMP-2) induced the ALP expression in pluripotent C3H10T1/2 cells, but it did not increase the transcriptional activity of the promoter region. Though they do not determine cell specific or BMP-2 inducible ALP expression, IRS and the E-box at position -241 seem to be important for the basic transcriptional activity of ALP gene.
|
| Volume |
44(4)
|
| Pages |
683-91
|
| Published |
1998-4-1
|
| DOI |
10.1080/15216549800201732
|
| PMID |
9584982
|
| MeSH |
3T3 Cells / metabolism
Alkaline Phosphatase / biosynthesis
Alkaline Phosphatase / genetics*
Animals
Bone Morphogenetic Protein 2
Bone Morphogenetic Proteins / pharmacology
Bone and Bones / enzymology*
Cell Line
Enhancer Elements, Genetic*
Exons*
Gene Expression / drug effects
Gene Expression Regulation, Enzymologic / drug effects
Kidney / enzymology*
Liver / enzymology*
Mice
Mice, Inbred ICR
Osteoblasts / enzymology
Osteoblasts / metabolism
Promoter Regions, Genetic
Transcription, Genetic
Transforming Growth Factor beta*
|
| Times Cited |
9
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
10T1/2(RCB0247)
NIH3TNIH3T3-3(RCB0150) |
