Reference - Detail
| RRC ID | 39246 |
|---|---|
| Author | Chen M, Shirai M, Liu Z, Arichi T, Takahashi H, Nishioka M. |
| Title | Efficient class II major histocompatibility complex presentation of endogenously synthesized hepatitis C virus core protein by Epstein-Barr virus-transformed B-lymphoblastoid cell lines to CD4(+) T cells. |
| Journal | J Virol |
| Abstract |
The induction of an efficient CD4(+) T-cell response against hepatitis C virus (HCV) is critical for control of the chronicity of HCV infection. The ability of HCV structural protein endogenously expressed in an antigen-presenting cell (APC) to be presented by class II major histocompatibility complex molecules to CD4(+) T cells was investigated by in vitro culture analyses using HCV core-specific T-cell lines and autologous Epstein-Barr virus-transformed B-lymphoblastoid cell lines (B-LCLs) expressing structural HCV antigens. The T- and B-cell lines were generated from peripheral blood mononuclear cells derived from HCV-infected patients. Expression and intracellular localization of core protein in transfected cells were determined by immunoblotting and immunofluorescence. By stimulation with autologous B-LCLs expressing viral antigens, strong T-cell proliferative responses were induced in two of three patients, while no substantial stimulatory effects were produced by B-LCLs expressing a control protein (chloramphenicol acetyltransferase) or by B-LCLs alone. The results showed that transfected B cells presented mainly endogenously synthesized core peptides. Presentation of secreted antigens from adjacent antigen-expressing cells was not enough to stimulate a core-specific T-cell response. Only weak T-cell proliferative responses were generated by stimulation with B-LCLs that had been pulsed beforehand with at least a 10-fold-higher amount of transfected COS cells in the form of cell lysate, suggesting that presentation of antigens released from dead cells in the B-LCL cultures had a minimal role. Titrating numbers of APCs, we showed that as few as 10(4) transfected B-LCL APCs were sufficient to stimulate T cells. This presentation pathway was found to be leupeptin sensitive, and it can be blocked by antibody to HLA class II (DR). In addition, expression of a costimulatory signal by B7/BB1 on B cells was essential for T-cell activation. |
| Volume | 72(10) |
| Pages | 8301-8 |
| Published | 1998-10-1 |
| DOI | 10.1128/JVI.72.10.8301-8308.1998 |
| PMID | 9733874 |
| PMC | PMC110194 |
| MeSH | Animals Antigen-Presenting Cells / immunology B-Lymphocytes / immunology* CD4-Positive T-Lymphocytes / immunology* COS Cells Cell Division / immunology Cell Line, Transformed Chloramphenicol O-Acetyltransferase / genetics Genes, Viral Herpesvirus 4, Human / physiology* Histocompatibility Antigens Class II / immunology* Leupeptins / immunology Viral Core Proteins / immunology* Viral Structural Proteins / genetics |
| IF | 4.501 |
| Times Cited | 31 |
| WOS Category | VIROLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 5 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | COS-7(RCB0539) |