| RRC ID |
39255
|
| 著者 |
Suzuki T, Yanagi K, Ookawa K, Hatakeyama K, Ohshima N.
|
| タイトル |
Blood flow and leukocyte adhesiveness are reduced in the microcirculation of a peritoneal disseminated colon carcinoma.
|
| ジャーナル |
Ann Biomed Eng
|
| Abstract |
Dynamic behavior of leukocytes in the microcirculation of solid tumor tissue was visualized using a fluorescent labeling technique combined with the use of a real-time confocal laser-scanning microscope (CLSM) system. Colon tumor cells (RCN-9) were inoculated into the peritoneal cavity of male Fischer 344 rats. Tumor-free rats were similarly injected with physiological saline (intraperitoneally). Ten days after tumor inoculation, the mesentery was exteriorized and subjected to vital microscopic observation under the CLSM system. Leukocytes were labeled with rhodamine 6G (100 microg kg(-1), intravenously), and their behavior within the microvessels (10-30 microm in diameter) was analyzed both in the solid tumor tissues and the normal mesentery. Wall shear rate was calculated from the measured values of vessel diameter and erythrocyte flow velocity. In tumor microvasculature of tumor-bearing rats, the centerline erythrocyte velocity (0.73 +/- 0.58 mm s(-1), mean +/- standard deviation) and wall shear rate (210 +/- 151 s(-1)) were significantly lower than those of the tumor-free rats (1.27 +/- 0.83 mm s(-1), 344 +/- 236 s(-1), respectively). Despite such reduced flow conditions, flux of the rolling leukocytes as well as density of the adhered leukocytes both decreased significantly in tumor microvasculature as compared with normal controls. The methods developed in this work show promise in improving our understanding of tumor biology and pathophysiology.
|
| 巻・号 |
26(5)
|
| ページ |
803-11
|
| 公開日 |
1998-1-1
|
| DOI |
10.1114/1.67
|
| PMID |
9779953
|
| MeSH |
Adhesiveness
Animals
Blood Flow Velocity*
Colonic Neoplasms / pathology*
Disease Models, Animal*
Hemorheology
Leukocytes / physiology*
Male
Microcirculation
Microscopy, Confocal
Neoplasms, Experimental*
Peritoneal Neoplasms / blood
Peritoneal Neoplasms / physiopathology*
Peritoneal Neoplasms / secondary*
Rats
Rats, Inbred F344
|
| IF |
3.324
|
| 引用数 |
16
|
|
WOS 分野
|
ENGINEERING, BIOMEDICAL
|
| リソース情報 |
| ヒト・動物細胞 |
RCN-9(RCB0511) |
