| RRC ID |
39259
|
| 著者 |
Aikawa J, Sato E, Kyuwa S, Sato E, Sasai K, Shiota K, Ogawa T.
|
| タイトル |
Asparagine-linked glycosylation of the rat leukemia inhibitory factor expressed by simian COS7 cells.
|
| ジャーナル |
Biosci Biotechnol Biochem
|
| Abstract |
The leukemia inhibitory factor (LIF) is a secretory glycoprotein and a pluripotent growth factor which acts in diverse cell systems. LIF has been reported to be heavily glycosylated. In this paper, we examine the transient expression of rat LIF (rLIF) in COS7 cells and its glycosylation by a PNGaseF treatment and lectin blot. rLIF expression in COS7 cells resulted in seven molecular species being produced with zero to six N-glycosyl moieties. Mutated rLIF proteins with substitutions at the seven possible N-glycosylation sites were also expressed. An analysis of the molecular weight of the mutated rLIF confirmed the six N-glycosylation sites. Bioassays of mouse leukemia cell lines were performed to analyze the contribution of the glycosyl moieties to their functions. We found that the glycosyl moieties at each of the N-glycosylation sites were not essential to their function of the protein, but the reduced functions to promote the proliferation of DA-1a cells that had been observed for some mutants suggests a biochemical role for the in vitro function.
|
| 巻・号 |
62(7)
|
| ページ |
1318-25
|
| 公開日 |
1998-7-1
|
| DOI |
10.1271/bbb.62.1318
|
| PMID |
9720213
|
| MeSH |
Amidohydrolases / chemistry
Amino Acid Sequence
Animals
Asparagine / chemistry*
Base Sequence
COS Cells
Cell Division / genetics
Glycosylation
Growth Inhibitors / biosynthesis*
Growth Inhibitors / chemistry
Growth Inhibitors / genetics
Haplorhini
Interleukin-6*
Lectins
Leukemia Inhibitory Factor
Lymphokines / biosynthesis*
Lymphokines / chemistry
Lymphokines / genetics
Mice
Molecular Sequence Data
Molecular Weight
Mutagenesis, Site-Directed
Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
Rats
Tumor Cells, Cultured
|
| IF |
1.516
|
| 引用数 |
6
|
|
WOS 分野
|
CHEMISTRY, APPLIED
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
