RRC ID 39290
著者 Otsuka E, Yamaguchi A, Hirose S, Hagiwara H.
タイトル Characterization of osteoblastic differentiation of stromal cell line ST2 that is induced by ascorbic acid.
ジャーナル Am J Physiol
Abstract The stromal cell line ST2, derived from mouse bone marrow, differentiated into osteoblast-like cells in response to ascorbic acid. Ascorbic acid induced alkaline phosphatase (ALPase) activity, the expression of mRNAs for proteins that are markers of osteoblastic differentiation, the deposition of calcium, and the formation of mineralized nodules by ST2 cells. We investigated the mechanism whereby ascorbic acid induced the differentiation of ST2 cells. Inhibitors of the formation of collagen triple helices completely blocked the effects of ascorbic acid on ST2 cells, an indication that matrix formation by type I collagen is essential for the induction of osteoblastic differentiation of ST2 cells by ascorbic acid. We furthermore examined the effects of bone morphogenetic proteins (BMPs) on the differentiation of ST2 cells induced by ascorbic acid. Ascorbic acid had no effect on the expression of mRNAs for BMP-4 and the BMP receptors. However, a soluble form of BMP receptor IA inhibited the induction of ALPase activity by ascorbic acid. These results suggest that ascorbic acid might promote the differentiation of ST2 cells into osteoblast-like cells by inducing the formation of a matrix of type I collagen, with subsequent activation of the signaling pathways that involve BMPs.
巻・号 277(1)
ページ C132-8
公開日 1999-7-1
DOI 10.1152/ajpcell.1999.277.1.C132
PMID 10409116
MeSH Alkaline Phosphatase / metabolism Animals Ascorbic Acid / pharmacology* Bone Morphogenetic Protein Receptors Bone Morphogenetic Proteins / metabolism Bone Morphogenetic Proteins / physiology Calcium / metabolism Cell Differentiation / drug effects Cell Differentiation / physiology Cell Line Collagen / pharmacology Mice Minerals / metabolism Osteoblasts / cytology* Receptors, Cell Surface / metabolism Receptors, Growth Factor* Stromal Cells / cytology* Stromal Cells / drug effects Stromal Cells / enzymology
IF 3.485
WOS 分野 PHYSIOLOGY CELL BIOLOGY
リソース情報
ヒト・動物細胞 ST2(RCB0224)