| RRC ID |
39297
|
| Author |
Yamasaki K, Sasaki T, Nemoto M, Eto Y, Tajima N.
|
| Title |
Differentiation-induced insulin secretion from nonendocrine cells with engineered human proinsulin cDNA.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
To investigate the effects of differentiation on insulin secretion from engineered nonendocrine cells, modified human proinsulin cDNA (INS/fur) was transfected to THP-1 monocyte and C2C12 myoblast cell lines. When THP-1 was differentiated into macrophages with phorbol ester, the insulin secretion rate was increased by 3.1-fold. This increase in insulin secretion is accompanied by a 17.6-fold increase in the processing efficiency of the modified human proinsulin and by a 3.5-fold increase in the abundance of furin mRNA. In addition, differentiation of C2C12 into myotubes, which can be induced by changing the serum, showed a 9.9-fold increase in insulin secretion and was accompanied by a 1.6-fold increase in the abundance of furin mRNA. The involvement of posttranslational processing and the exocytotic process in differentiation-induced insulin secretion could lead to the possibility of regulation of insulin secretion from genetically engineered cells.
|
| Volume |
265(2)
|
| Pages |
361-5
|
| Published |
1999-11-19
|
| DOI |
10.1006/bbrc.1999.1698
|
| PII |
S0006-291X(99)91698-4
|
| PMID |
10558872
|
| MeSH |
3T3 Cells
Animals
Cell Differentiation / genetics
Cell Differentiation / physiology*
Cell Line
DNA, Complementary / genetics*
Diabetes Mellitus, Type 1 / genetics
Diabetes Mellitus, Type 1 / physiopathology
Diabetes Mellitus, Type 1 / therapy
Furin
Genetic Engineering
Genetic Therapy
Humans
Insulin / metabolism*
Insulin Secretion
Mice
Proinsulin / genetics*
Proinsulin / metabolism
Protein Processing, Post-Translational
RNA, Messenger / genetics
RNA, Messenger / metabolism
Subtilisins / genetics
Subtilisins / metabolism
Transfection
|
| IF |
2.985
|
| Times Cited |
12
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
C2C12(RCB0987) |
