論文 - 詳細
| RRC ID | 39305 |
|---|---|
| 著者 | Kitazawa R, Kitazawa S, Maeda S. |
| タイトル | Promoter structure of mouse RANKL/TRANCE/OPGL/ODF gene. |
| ジャーナル | Biochim Biophys Acta |
| Abstract |
Receptor activator of NF-kappa B ligand (RANKL)/tumor necrosis factor-related activation induced cytokine (TRANCE)/osteoprotegerin ligand (OPGL)/osteoclast differentiation factor (ODF) is a membrane-bound signal transducer responsible for differentiation and maintenance of osteoclasts. To elucidate the mechanism regulating RANKL/TRANCE/OPGL/ODF gene expression, we cloned the 5'-flanking basic promoter region of the mouse RANKL/TRANCE/OPGL/ODF gene and characterized it by transient transfection studies and genomic Southern blot analysis. Inverted TATA- and CAAT-boxes and a putative Cbfa1/Osf2/AML3 binding domain constituted the basic promoter structure. The repeated half-sites for the vitamin D3 (VitD3) and glucocorticoid receptors were located at -935 and -640, respectively. Transient transfection studies revealed that short-term treatment with 1alpha,25(OH)2 VitD3 or dexamethasone increased luciferase activity up to 204% and 178%, respectively; on the other hand, treatment with dibutyryl cyclic AMP did not affect the promoter activity. Since the expression of Cbfa1/Osf2/AML3 is also regulated by VitD3, 1alpha,25(OH)2 VitD3 might affect RANKL/TRANCE/OPGL/ODF gene expression both directly and indirectly. CpG methylation was observed dominantly in mouse stromal cells, ST2, of a later passage which ceased to support in vitro osteoclastogenesis, suggesting that the methylation status of the CpG loci in the RANKL/TRANCE/OPGL/ODF gene promoter may be one of the influential cis-regulating factors. |
| 巻・号 | 1445(1) |
| ページ | 134-41 |
| 公開日 | 1999-4-14 |
| DOI | 10.1016/s0167-4781(99)00032-9 |
| PII | S0167-4781(99)00032-9 |
| PMID | 10209265 |
| MeSH | Animals Base Sequence Blotting, Southern Carrier Proteins / genetics* Cloning, Molecular Coculture Techniques Hypercalcemia / genetics Membrane Glycoproteins / genetics* Mice Molecular Sequence Data Osteoclasts / metabolism Osteoporosis / genetics Promoter Regions, Genetic* RANK Ligand Receptor Activator of Nuclear Factor-kappa B Transfection |
| IF | 3.411 |
| 引用数 | 119 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 10 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | ST2(RCB0224) |