Reference - Detail
| RRC ID | 39309 |
|---|---|
| Author | Tani K, Mizoguchi T, Iwamatsu A, Hatsuzawa K, Tagaya M. |
| Title | p125 is a novel mammalian Sec23p-interacting protein with structural similarity to phospholipid-modifying proteins. |
| Journal | J Biol Chem |
| Abstract |
COPII-coated vesicles are involved in protein transport from the endoplasmic reticulum to the Golgi apparatus. COPII consists of three parts: Sar1p and the two protein complexes, Sec23p-Sec24p and Sec13p-Sec31p. Using a glutathione S-transferase fusion protein with mouse Sec23p, we identified a novel mammalian Sec23p-interacting protein, p125, which is clearly distinct from Sec24p. The N-terminal region of p125 is rich in proline residues, and the central and C-terminal regions exhibit significant homology to phospholipid-modifying proteins, especially phosphatidic acid preferring-phospholipase A1. We transiently expressed p125 and mouse Sec23p in mammalian cells and examined their interaction. The results showed that the N-terminal region of p125 is important for the interaction with Sec23p. We confirmed the interaction between the two proteins by a yeast two-hybrid assay. Overexpression of p125, like that of mammalian Sec23p, caused disorganization of the endoplasmic reticulum-Golgi intermediate compartment and Golgi apparatus, suggesting its role in the early secretory pathway. |
| Volume | 274(29) |
| Pages | 20505-12 |
| Published | 1999-7-16 |
| DOI | 10.1074/jbc.274.29.20505 |
| PII | S0021-9258(19)72680-5 |
| PMID | 10400679 |
| MeSH | Amino Acid Sequence Animals Base Sequence Blotting, Northern Carrier Proteins / chemistry Carrier Proteins / metabolism* Cell Line Chlorocebus aethiops Cloning, Molecular DNA Primers DNA, Complementary Golgi Apparatus / metabolism Humans Mice Molecular Sequence Data Phosphatidic Acids / metabolism Phospholipases A / metabolism* Phospholipids / chemistry* Protein Binding Proteins / genetics Proteins / metabolism* RNA-Binding Proteins Sequence Homology, Amino Acid Vero Cells Vesicular Transport Proteins |
| IF | 4.238 |
| Times Cited | 76 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 6 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | Vero(RCB0001) NRK(RCB0043) |