RRC ID 3931
著者 Hozumi A, Padma P, Toda T, Ide H, Inaba K.
タイトル Molecular characterization of axonemal proteins and signaling molecules responsible for chemoattractant-induced sperm activation in Ciona intestinalis.
ジャーナル Cell Motil Cytoskeleton
Abstract Spermatozoa undergo dramatic physiological changes at fertilization. In the ascidian Ciona intestinalis, an egg-derived substance named SAAF induces both sperm activation and chemotaxis to the egg. To elucidate the molecular mechanism underlying these phenomena, whole sperm proteins before and after SAAF-treatment were analyzed by two-dimensional gel electrophoresis. By comparison of spot patterns before and after activation, we found twelve proteins that changed the isoelectric points. Seven proteins were shown to be axonemal proteins and others were suggested to be non-axonemal components. Analysis of these proteins by MS-based proteomic system revealed that components of several substructures of the axonemes underwent the changes in isoelectric point at sperm activation, including WD-repeat intermediate chains of outer and inner arm dyneins and a radial spoke protein LRR37, as well as novel axonemal proteins with armadillo repeats or SMC domain. Molecules for cell signaling such as 14-3-3 proteins, Skp1 and VCP/p97 also showed isoelectric changes at sperm activation. These results show a comprehensive feature for signaling mechanism of the activation of spermatozoa at fertilization and also shed new lights on the regulation of ciliary and flagellar movements.
巻・号 65(3)
ページ 249-67
公開日 2008-3-1
DOI 10.1002/cm.20258
PMID 18189282
MeSH 14-3-3 Proteins / metabolism Animals Axoneme / drug effects Axoneme / metabolism* Blotting, Western Chemotactic Factors / pharmacology* Ciona intestinalis / cytology* Ciona intestinalis / drug effects* Ciona intestinalis / metabolism Dyneins / chemistry Dyneins / metabolism Electrophoresis, Gel, Two-Dimensional Male Peptide Mapping Phosphorylation / drug effects Phylogeny Protein Transport / drug effects Proteins / metabolism Signal Transduction* / drug effects Sperm Motility / drug effects* Spermatozoa / cytology Spermatozoa / drug effects* Spermatozoa / metabolism
引用数 28
WOS 分野 CELL BIOLOGY
リソース情報
カタユウレイボヤ・(ニッポンウミシダ) Wild C.int?