| RRC ID |
39319
|
| 著者 |
Fujiwara M, Tagashira S, Harada H, Ogawa S, Katsumata T, Nakatsuka M, Komori T, Takada H.
|
| タイトル |
Isolation and characterization of the distal promoter region of mouse Cbfa1.
|
| ジャーナル |
Biochim Biophys Acta
|
| Abstract |
Cbfa1 is an essential transcription factor for bone formation, and as such little is known about the region responsible for the transcriptional regulation of this gene. Here we report the determination of the transcription start sites, isolation and partial characterization of distal promoter region of this gene. Three transcription start sites were identified by the 5'-Cap site method, recently invented for rapid examination of the 5'-end of genes of interest. A reporter construct containing 1.8 kb of 5' of transcription start sites had approximately 25-fold more luciferase activity than the promoter-less vector in osteoblastic cell lines. Deletion analysis of the reporter construct demonstrated that the minimal region to express promoter activity lies between bp -168 and -99, taking the most downstream transcription start site as +1. By Northern blot analysis, mRNA expression from the distal promoter was detected in the differentiated osteoblastic cell lines, UMR-106, ROS17/2.8 and MC3T3-E1, but not in cell lines of immature phenotype or originated from other organs. Luciferase activity was strongest in UMR-106 and ROS17/2.8, and weakest in COS-1 and HepG2, which are cell lines originating from other organs, corresponding to the level of mRNA expression. These results demonstrated that the distal promoter region examined here is important for tissue- and cell-type-specific gene expression of Cbfa1.
|
| 巻・号 |
1446(3)
|
| ページ |
265-72
|
| 公開日 |
1999-9-3
|
| DOI |
10.1016/s0167-4781(99)00113-x
|
| PII |
S0167-4781(99)00113-X
|
| PMID |
10524201
|
| MeSH |
Animals
Base Sequence
Cell Line
Cloning, Molecular
Core Binding Factor Alpha 1 Subunit
DNA-Binding Proteins / genetics*
Haplorhini
Humans
Mice
Molecular Sequence Data
Neoplasm Proteins*
Plasmids
Polymerase Chain Reaction
Promoter Regions, Genetic
RNA, Messenger / genetics
RNA, Messenger / isolation & purification
Rats
Transcription Factors / genetics*
Transfection
|
| IF |
3.411
|
| 引用数 |
43
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-G2/PA6(RCB1127)
ST2(RCB0224) |
