| RRC ID |
39323
|
| 著者 |
Ueda Y, Bandoh S, Fujita J, Sato M, Yamaji Y, Takahara J.
|
| タイトル |
Expression of nerve growth factor-induced clone B subfamily and pro-opiomelanocortin gene in lung cancer cell lines.
|
| ジャーナル |
Am J Respir Cell Mol Biol
|
| Abstract |
Nerve growth factor-induced clone B (NGFI-B), Nur-related factor 1, and neuron-derived orphan receptor-1 have structural features of ligand-activated transcriptional regulators and constitute the NGFI-B subfamily within the nuclear receptor superfamily. The NGFI-B subfamily is highly expressed in neuroendocrine organs and regulates the pro-opiomelanocortin (POMC) gene. Small-cell lung cancer (SCLC) is considered to be a neuroendocrine tumor that produces large numbers of polypeptide hormones. In this study we measured the NGFI-B subfamily and POMC messenger RNA (mRNA) levels in various lung-cancer cell lines by means of the quantitative reverse transcription-polymerase chain reaction, and evaluated the correlations between expression of these genes and polypeptide hormone productions. We also examined the effect of antisense oligonucleotide to NGFI-B mRNA on the expression of POMC mRNA. The NGFI-B subfamily and POMC mRNAs were highly expressed in SCLC cell lines. In addition, there were strong correlations between the NGFI-B, POMC genes, and the adrenocorticotropin hormone (ACTH) level. Further, the antisense oligonucleotide significantly suppressed POMC gene expression. We conclude that the NGFI-B subfamily was a significant molecule in SCLC and that the NGFI-B was a positive transcriptional factor for ACTH production.
|
| 巻・号 |
20(6)
|
| ページ |
1319-25
|
| 公開日 |
1999-6-1
|
| DOI |
10.1165/ajrcmb.20.6.3577
|
| PMID |
10340952
|
| MeSH |
Carcinoma, Non-Small-Cell Lung / metabolism*
Carcinoma, Small Cell / metabolism*
DNA-Binding Proteins / metabolism*
Dose-Response Relationship, Drug
Gene Expression
Humans
Lung Neoplasms / metabolism*
Multigene Family
Nerve Growth Factors / metabolism*
Nerve Tissue Proteins / metabolism
Nuclear Receptor Subfamily 4, Group A, Member 1
Nuclear Receptor Subfamily 4, Group A, Member 2
Oligonucleotides, Antisense
Pro-Opiomelanocortin / genetics*
Pro-Opiomelanocortin / metabolism
Receptors, Cytoplasmic and Nuclear
Receptors, Steroid
Receptors, Thyroid Hormone
Transcription Factors / metabolism*
Tumor Cells, Cultured
|
| IF |
5.373
|
| 引用数 |
10
|
|
WOS 分野
|
RESPIRATORY SYSTEM
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
Lu-139(RCB0469)
Lu-165(RCB1184) |
