論文 - 詳細
| RRC ID | 39323 |
|---|---|
| 著者 | Ueda Y, Bandoh S, Fujita J, Sato M, Yamaji Y, Takahara J. |
| タイトル | Expression of nerve growth factor-induced clone B subfamily and pro-opiomelanocortin gene in lung cancer cell lines. |
| ジャーナル | Am J Respir Cell Mol Biol |
| Abstract |
Nerve growth factor-induced clone B (NGFI-B), Nur-related factor 1, and neuron-derived orphan receptor-1 have structural features of ligand-activated transcriptional regulators and constitute the NGFI-B subfamily within the nuclear receptor superfamily. The NGFI-B subfamily is highly expressed in neuroendocrine organs and regulates the pro-opiomelanocortin (POMC) gene. Small-cell lung cancer (SCLC) is considered to be a neuroendocrine tumor that produces large numbers of polypeptide hormones. In this study we measured the NGFI-B subfamily and POMC messenger RNA (mRNA) levels in various lung-cancer cell lines by means of the quantitative reverse transcription-polymerase chain reaction, and evaluated the correlations between expression of these genes and polypeptide hormone productions. We also examined the effect of antisense oligonucleotide to NGFI-B mRNA on the expression of POMC mRNA. The NGFI-B subfamily and POMC mRNAs were highly expressed in SCLC cell lines. In addition, there were strong correlations between the NGFI-B, POMC genes, and the adrenocorticotropin hormone (ACTH) level. Further, the antisense oligonucleotide significantly suppressed POMC gene expression. We conclude that the NGFI-B subfamily was a significant molecule in SCLC and that the NGFI-B was a positive transcriptional factor for ACTH production. |
| 巻・号 | 20(6) |
| ページ | 1319-25 |
| 公開日 | 1999-6-1 |
| DOI | 10.1165/ajrcmb.20.6.3577 |
| PMID | 10340952 |
| MeSH | Carcinoma, Non-Small-Cell Lung / metabolism* Carcinoma, Small Cell / metabolism* DNA-Binding Proteins / metabolism* Dose-Response Relationship, Drug Gene Expression Humans Lung Neoplasms / metabolism* Multigene Family Nerve Growth Factors / metabolism* Nerve Tissue Proteins / metabolism Nuclear Receptor Subfamily 4, Group A, Member 1 Nuclear Receptor Subfamily 4, Group A, Member 2 Oligonucleotides, Antisense Pro-Opiomelanocortin / genetics* Pro-Opiomelanocortin / metabolism Receptors, Cytoplasmic and Nuclear Receptors, Steroid Receptors, Thyroid Hormone Transcription Factors / metabolism* Tumor Cells, Cultured |
| IF | 5.373 |
| 引用数 | 10 |
| WOS 分野 | RESPIRATORY SYSTEM BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Lu-139(RCB0469) Lu-165(RCB1184) |