RRC ID 39326
著者 Kobori M, Iwashita K, Shinmoto H, Tsushida T.
タイトル Phloretin-induced apoptosis in B16 melanoma 4A5 cells and HL60 human leukemia cells.
ジャーナル Biosci Biotechnol Biochem
Abstract The dihydrochalcone phloretin induced apoptosis in B16 mouse melanoma 4A5 cells and HL60 human leukemia cells. Phloretin was suggested to induce apoptosis in B16 cells mainly through the inhibition of glucose transmembrane transport. The phloretin-induced apoptosis in B16 cells was inhibited by actinomycin D, Ac-YVAD-CHO caspase-1-like inhibitor, and Ac-DEVD-CHO caspase-3-like inhibitor. During the induction of apoptosis by phloretin, the expression of Bax protein in B16 cells increased and the levels of p53, Bcl-2, and Bcl-XL proteins did not change. Our results suggested that phloretin induced apoptosis through the promotion of Bax protein expression and caspases activation. On the other hand, phloretin may induce apoptosis in HL60 cells through the inhibition of protein kinase C activity because phloretin inhibited protein kinase C activity in HL60 cells more than that in B16 cells. The phloretin induced-apoptosis in HL60 cells was not inhibited by actinomycin D and the caspase-1-like inhibitor, but slightly inhibited by the caspase-3-like inhibitor. Phloretin reduced the level of caspase 3 protein in HL60 cells, but not the level of the Bcl-2 protein. Phloretin did not increase the level of Bax protein. Phloretin was suggested to induce apoptosis in HL60 cells through the inhibition of protein kinase C activity, followed by the pathway, which is different from that in B16 cells.
巻・号 63(4)
ページ 719-25
公開日 1999-4-1
DOI 10.1271/bbb.63.719
PMID 10361685
MeSH Animals Antibodies, Monoclonal Apoptosis / drug effects* Blotting, Western CHO Cells Cell Nucleus / drug effects Cell Nucleus / ultrastructure Cricetinae Electrophoresis, Agar Gel Flow Cytometry HL-60 Cells Humans Melanoma, Experimental / drug therapy Melanoma, Experimental / pathology Mice Phloretin / antagonists & inhibitors Phloretin / pharmacology* Protein Kinase C / metabolism Protein Synthesis Inhibitors / pharmacology
IF 1.516
引用数 58
WOS 分野 CHEMISTRY, APPLIED FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 B16 melanoma 4A5(RCB0557)