| RRC ID |
39331
|
| 著者 |
Yoshinaga H, Matsuhashi S, Fujiyama C, Masaki Z.
|
| タイトル |
Novel human PDCD4 (H731) gene expressed in proliferative cells is expressed in the small duct epithelial cells of the breast as revealed by an anti-H731 antibody.
|
| ジャーナル |
Pathol Int
|
| Abstract |
The novel gene H731 (approved name: PDCD4 (programmed cell death 4)) has been isolated as an antigen gene of the monoclonal antibody Pr-28 which recognized a nuclear antigen in proliferating cells. The gene is homologous to the mouse gene (MA-3/Pdcd4/A7-1) which was associated with apoptosis and was shown to suppress tumor promoter-induced neoplastic transformation. A polyclonal antibody against H731-protein derived from an extract of Escherichia coli transformed with an H731 expression plasmid was prepared, and the H731-protein expression in human normal and tumor cells using the antibody was studied. The staining patterns of asynchronous cultures of human normal fibroblasts (MRC-5) were heterogeneous but the antigen was accumulated in the nuclei at the G0 phase. On the contrary, the antigen was overproduced and localized in the cytoplasm during the cell cycle in tumor cell lines. Immunohistological studies revealed that the H731-protein was highly expressed in bladder carcinoma and breast carcinoma tissues compared with the normal tissues so far tested. These results indicated that expression of the H731-protein was up-regulated or induced in the proliferative cells. Immunohistological studies also revealed that the protein was abundantly expressed in the small duct epithelial cells of the normal mammary gland.
|
| 巻・号 |
49(12)
|
| ページ |
1067-77
|
| 公開日 |
1999-12-1
|
| DOI |
10.1046/j.1440-1827.1999.00995.x
|
| PII |
pin995
|
| PMID |
10632927
|
| MeSH |
Amino Acid Sequence
Apoptosis
Apoptosis Regulatory Proteins
Blotting, Western
Breast / metabolism*
Breast Neoplasms / genetics*
Breast Neoplasms / metabolism
Carcinoma in Situ / genetics*
Carcinoma in Situ / metabolism
Carcinoma, Ductal, Breast / genetics*
Carcinoma, Ductal, Breast / metabolism
Cell Division
Cell Line, Transformed / metabolism
Epithelial Cells / metabolism*
Escherichia coli / genetics
Female
Fibroblasts / metabolism
Gene Expression*
Humans
Immunohistochemistry
Molecular Sequence Data
Protein Biosynthesis
Proteins / genetics*
Proteins / immunology
Proteins / isolation & purification
RNA-Binding Proteins*
Tumor Cells, Cultured
|
| IF |
2.11
|
| 引用数 |
52
|
|
WOS 分野
|
PATHOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
MRC-5 SV1 TG1(RCB0207) |
