Reference - Detail
| RRC ID | 39335 |
|---|---|
| Author | Nukaya I, Yasumoto M, Iwasaki T, Ideno M, Sette A, Celis E, Takesako K, Kato I. |
| Title | Identification of HLA-A24 epitope peptides of carcinoembryonic antigen which induce tumor-reactive cytotoxic T lymphocyte. |
| Journal | Int J Cancer |
| Abstract |
Carcinoembryonic antigen (CEA), which is expressed in several cancer types, is a potential target for specific immunotherapy. HLA-A24 is the most frequent allele among Japanese and is also frequently present in Asians and Caucasians. We tested CEA-encoded HLA-A24 binding peptides for their capacity to elicit anti-tumor cytotoxic T lymphocytes (CTL) in vitro. For this purpose, we used CD8+ T lymphocytes from peripheral blood mononuclear cells (PBMC) of a healthy donor and autologous peptide-pulsed dendritic cells as antigen-presenting cells. This approach enabled us to identify 2 peptides, QYSWFVNGTF and TYACFVSNL, which were capable of eliciting CTL lines that lysed tumor cells expressing HLA-A24 and CEA. The cytotoxicity to tumor cells by the CTL lines was antigen-specific since it was inhibited by peptide-pulsed cold target cells as well as by anti-class I major histocompatibility complex (MHC) and anti-CD3 monoclonal antibodies (MAbs). The antigen specificity of the 2 CTL lines was examined using several tumor cell lines of various origins and for their peptide-dose responses. The identification of these novel CEA epitopes for CTL offers the opportunity to design and develop epitope-based immunotherapeutic approaches for treating HLA-A24+ patients with tumors that express CEA. |
| Volume | 80(1) |
| Pages | 92-7 |
| Published | 1999-1-5 |
| DOI | 10.1002/(sici)1097-0215(19990105)80:1<92::aid-ijc18>3.0.co;2-m |
| PII | 10.1002/(SICI)1097-0215(19990105)80:1<92::AID-IJC18>3.0.CO;2-M |
| PMID | 9935237 |
| MeSH | Amino Acid Sequence Antibodies, Monoclonal / pharmacology Asian People / genetics Carcinoembryonic Antigen / chemistry Carcinoembryonic Antigen / genetics* Cytotoxicity, Immunologic* Dendritic Cells / immunology Digestive System Neoplasms / immunology Epitopes / chemistry Epitopes / genetics Epitopes / immunology HLA-A Antigens / genetics* HLA-A Antigens / immunology* HLA-A24 Antigen Histocompatibility Antigens Class I / immunology Humans Interferon-gamma / pharmacology Japan Lymphoma, B-Cell / immunology Melanoma / immunology T-Lymphocytes, Cytotoxic / immunology* Tumor Cells, Cultured White People / genetics |
| IF | 5.145 |
| Times Cited | 90 |
| WOS Category | ONCOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 105 |
| Altmetric score changes over past 6months | 3.0 |
| Resource | |
| Human and Animal Cells | GT3TKB(RCB0885) |