論文 - 詳細
| RRC ID | 39345 |
|---|---|
| 著者 | Murata-Hori M, Suizu F, Iwasaki T, Kikuchi A, Hosoya H. |
| タイトル | ZIP kinase identified as a novel myosin regulatory light chain kinase in HeLa cells. |
| ジャーナル | FEBS Lett |
| Abstract |
A novel myosin light chain kinase (MLCK) cDNA was isolated from a HeLa cell cDNA library. The deduced amino acid sequence was identical to that of a zipper-interacting protein kinase (ZIPK) which mediates apoptosis [Kawai et al. (1998) Mol. Cell. Biol. 18, 1642-1651]. Here we found that HeLa ZIPK phosphorylated the regulatory light chain of myosin II (MRLC) at both serine 19 and threonine 18 in a Ca2+/calmodulin independent manner. Phosphorylation of myosin II by HeLa ZIPK resulted in activation of actin-activated MgATPase activity of myosin II. HeLa ZIPK is the first non-muscle MLCK that phosphorylates MRLC at two sites. |
| 巻・号 | 451(1) |
| ページ | 81-4 |
| 公開日 | 1999-5-14 |
| DOI | 10.1016/s0014-5793(99)00550-5 |
| PII | S0014-5793(99)00550-5 |
| PMID | 10356987 |
| MeSH | Amino Acid Sequence Apoptosis Regulatory Proteins Base Sequence Calcium-Calmodulin-Dependent Protein Kinases DNA, Complementary Death-Associated Protein Kinases HeLa Cells Humans Molecular Sequence Data Myosin-Light-Chain Kinase / metabolism* Phosphorylation Protein Serine-Threonine Kinases / genetics Protein Serine-Threonine Kinases / metabolism* |
| IF | 3.057 |
| 引用数 | 109 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) |