| RRC ID |
39348
|
| 著者 |
Miyauchi M, Shimada H, Kadomatsu K, Muramatsu T, Matsubara S, Ikematsu S, Takenaga K, Asano T, Ochiai T, Sakiyama S, Tagawa M.
|
| タイトル |
Frequent expression of midkine gene in esophageal cancer suggests a potential usage of its promoter for suicide gene therapy.
|
| ジャーナル |
Jpn J Cancer Res
|
| Abstract |
We have examined the expression of midkine (MK), a neurotrophic factor with heparin-binding activity, in human esophageal cancer cells. Seven esophageal cell lines tested expressed the transcript and 8 out of 14 human esophageal tumor specimens were positively stained with anti-MK antibody, while surrounding normal esophageal tissues in these specimens were not stained. The 5'-flanking, 2.3 kb genomic region of the MK gene was shown to drive the transcription of a reporter gene in the esophageal cell lines in a cis acting manner. Forced expression in esophageal cancer cells of herpes simplex virus-thymidine kinase gene mediated by the flanking region of the MK gene conferred sensitivity to a prodrug, ganciclovir. The 5'-upstream region of the MK gene thus possesses putative promoter activity which can be used for suicide gene-based gene therapy for esophageal cancer.
|
| 巻・号 |
90(4)
|
| ページ |
469-75
|
| 公開日 |
1999-4-1
|
| DOI |
10.1111/j.1349-7006.1999.tb00771.x
|
| PII |
S0910505099800878
|
| PMID |
10363587
|
| PMC |
PMC5926091
|
| MeSH |
Aged
Antiviral Agents / therapeutic use
Carrier Proteins / genetics*
Cytokines*
Esophageal Neoplasms / therapy*
Female
Ganciclovir / therapeutic use
Gene Expression Regulation, Neoplastic / physiology*
Gene Transfer Techniques
Genetic Therapy / methods*
Humans
Immunohistochemistry
Male
Middle Aged
Midkine
Neoplasm Proteins / genetics*
Nerve Growth Factors / genetics*
Promoter Regions, Genetic
Tumor Cells, Cultured
|
| 引用数 |
40
|
|
WOS 分野
|
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
EC-GI-10(RCB0774) |
