論文 - 詳細
| RRC ID | 39348 |
|---|---|
| 著者 | Miyauchi M, Shimada H, Kadomatsu K, Muramatsu T, Matsubara S, Ikematsu S, Takenaga K, Asano T, Ochiai T, Sakiyama S, Tagawa M. |
| タイトル | Frequent expression of midkine gene in esophageal cancer suggests a potential usage of its promoter for suicide gene therapy. |
| ジャーナル | Jpn J Cancer Res |
| Abstract |
We have examined the expression of midkine (MK), a neurotrophic factor with heparin-binding activity, in human esophageal cancer cells. Seven esophageal cell lines tested expressed the transcript and 8 out of 14 human esophageal tumor specimens were positively stained with anti-MK antibody, while surrounding normal esophageal tissues in these specimens were not stained. The 5'-flanking, 2.3 kb genomic region of the MK gene was shown to drive the transcription of a reporter gene in the esophageal cell lines in a cis acting manner. Forced expression in esophageal cancer cells of herpes simplex virus-thymidine kinase gene mediated by the flanking region of the MK gene conferred sensitivity to a prodrug, ganciclovir. The 5'-upstream region of the MK gene thus possesses putative promoter activity which can be used for suicide gene-based gene therapy for esophageal cancer. |
| 巻・号 | 90(4) |
| ページ | 469-75 |
| 公開日 | 1999-4-1 |
| DOI | 10.1111/j.1349-7006.1999.tb00771.x |
| PII | S0910505099800878 |
| PMID | 10363587 |
| PMC | PMC5926091 |
| MeSH | Aged Antiviral Agents / therapeutic use Carrier Proteins / genetics* Cytokines* Esophageal Neoplasms / therapy* Female Ganciclovir / therapeutic use Gene Expression Regulation, Neoplastic / physiology* Gene Transfer Techniques Genetic Therapy / methods* Humans Immunohistochemistry Male Middle Aged Midkine Neoplasm Proteins / genetics* Nerve Growth Factors / genetics* Promoter Regions, Genetic Tumor Cells, Cultured |
| 引用数 | 40 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | EC-GI-10(RCB0774) |