論文 - 詳細
| RRC ID | 39349 |
|---|---|
| 著者 | Sueoka E, Goto Y, Sueoka N, Kai Y, Kozu T, Fujiki H. |
| タイトル | Heterogeneous nuclear ribonucleoprotein B1 as a new marker of early detection for human lung cancers. |
| ジャーナル | Cancer Res |
| Abstract |
Heterogeneous nuclear ribonucleoprotein (hnRNP) A2/B1 is an RNA binding protein that is required for maturation of mRNA precursor. Tockman et al. previously reported that hnRNP A2/B1 with a M(r) of 31,000 is overexpressed from the early clinical stage of human lung cancer (M. S. Tockman et al., J. Clin. Oncol., 6: 1685-1693, 1988). However, when hnRNP A2/B1 mRNA and hnRNP B1 mRNA were separately studied, we found unique evidence that hnRNP B1 mRNA, which is a splicing variant of hnRNP A2 mRNA, was more significantly elevated in lung cancer tissues than hnRNP A2/B1 mRNA. Our hnRNP B1-specific polyclonal antibody specifically recognized hnRNP B1 protein as a M(r) 37,000 nuclear protein by Western blotting but did not recognize hnRNP A2 protein. Immunohistochemical staining with the hnRNP B1 antibody revealed that hnRNP B1 protein was specifically stained in the nuclei of human cancer cells, and in squamous cell carcinomas in particular, but not in those of normal adjacent lung epithelial cells. We think that hnRNP B1 protein of M(r) 37,000, not hnRNP A2, is well qualified as a biomarker for the detection of human lung cancer. |
| 巻・号 | 59(7) |
| ページ | 1404-7 |
| 公開日 | 1999-4-1 |
| PMID | 10197602 |
| MeSH | Biomarkers, Tumor / analysis* Epithelial Cells / metabolism Heterogeneous-Nuclear Ribonucleoprotein Group A-B* Heterogeneous-Nuclear Ribonucleoproteins Humans Immunohistochemistry Lung / metabolism Lung Neoplasms / diagnosis* RNA, Messenger / analysis Ribonucleoproteins / analysis* Ribonucleoproteins / genetics Tumor Cells, Cultured |
| IF | 9.727 |
| 引用数 | 91 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 15 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | A549(RCB0098) |