論文 - 詳細
| RRC ID | 39355 |
|---|---|
| 著者 | Mizuki E, Ohba M, Akao T, Yamashita S, Saitoh H, Park YS. |
| タイトル | Unique activity associated with non-insecticidal Bacillus thuringiensis parasporal inclusions: in vitro cell-killing action on human cancer cells. |
| ジャーナル | J Appl Microbiol |
| Abstract |
Parasporal inclusion proteins from a total of 1744 Bacillus thuringiensis strains, consisting of 1700 Japanese isolates and 44 reference type strains of existing H serovars, were screened for cytocidal activity against human leukaemia T cells and haemolytic activity against sheep erythrocytes. Of 1684 B. thuringiensis strains having no haemolytic activity, 42 exhibited in vitro cytotoxicity against leukaemia T cells. These non-haemolytic but leukaemia cell-toxic strains belonged to several H-serovars including dakota, neoleonensis, shandongiensis, coreanensis and other unidentified serogroups. Purified parasporal inclusions of the three selected strains, designated 84-HS-1-11, 89-T-26-17 and 90-F-45-14, exhibited no haemolytic activity and no insecticidal activity against dipteran and lepidopteran insects, but were highly cytocidal against leukaemia T cells and other human cancer cells, showing different toxicity spectra and varied activity levels. Furthermore, the proteins from 84-HS-1-11 and 89-T-26-17 were able to discriminate between leukaemia and normal T cells, specifically killing the former cells. These findings may lead to the use of B. thuringiensis inclusion proteins for medical purposes. |
| 巻・号 | 86(3) |
| ページ | 477-86 |
| 公開日 | 1999-3-1 |
| DOI | 10.1046/j.1365-2672.1999.00692.x |
| PMID | 10196753 |
| MeSH | Animals Antineoplastic Agents / pharmacology* Bacillus thuringiensis / chemistry* Bacterial Proteins / analysis Bacterial Proteins / pharmacology* Bombyx / drug effects Cockroaches / drug effects Diptera / drug effects Electrophoresis, Polyacrylamide Gel Erythrocytes / drug effects Fibroblasts / drug effects HeLa Cells Humans Insecticides / pharmacology Isoptera / drug effects Leukemia, T-Cell Lung Neoplasms Orthoptera / drug effects Spores, Bacterial / chemistry* |
| IF | 3.066 |
| 引用数 | 114 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MOLT-4(RCB0206) A549(RCB0098) MRC-5 HeLa(RCB0007) GAKS(RCB1452) BM-N(RCB0457) NIAS-AeAl-2(RCB0277) |