| RRC ID |
39359
|
| 著者 |
Tsuji T, Han SA, Takeuchi K, Takahashi N, Hakomori S, Irimura T.
|
| タイトル |
Characterization of mouse integrin alpha3 subunit gene.
|
| ジャーナル |
J Biochem
|
| Abstract |
Integrin alpha3beta1 (VLA-3) is an adhesion receptor for extracellular matrix proteins including various isoforms of laminin. We have isolated mouse genomic clones encoding the integrin alpha3 subunit and deduced the exon/intron organization. The mouse integrin alpha3 subunit gene is encoded by 26 exons spanning 40 kb. The exon/intron structure of the integrin alpha3 subunit gene resembles that of the integrin alpha6 subunit gene, but differs somewhat from those of other members of the integrin family. We have demonstrated that the cytoplasmic domain splicing variants of the alpha3 subunits (alpha3A and alpha3B) are generated by alternative exon usage. We also cloned the 5'-flanking region and performed a preliminary analysis of its promoter activity in various tumor cell lines with different degrees of integrin alpha3 expression. Following transfection, activity in the luciferase assay was found to be roughly correlated with the expression level of integrin alpha3 as measured by flow cytometry. Furthermore, the luciferase assay was performed with normal and SV-40- or polyoma virus-transformed fibroblasts. In mouse, human, and hamster fibroblasts, higher levels of luciferase expression were observed in transformed cells than in normal cells. This result is consistent with our previous finding that integrin alpha3 expression at both the protein and mRNA levels is enhanced upon oncogenic transformation of fibroblasts by tumor viruses.
|
| 巻・号 |
125(6)
|
| ページ |
1183-8
|
| 公開日 |
1999-6-1
|
| DOI |
10.1093/oxfordjournals.jbchem.a022402
|
| PMID |
10348923
|
| MeSH |
Alternative Splicing
Amino Acid Sequence
Animals
Antigens, CD / genetics*
Base Sequence
Cell Line
Cell Transformation, Neoplastic
Cloning, Molecular
Cricetinae
DNA / genetics
Exons
Gene Expression
Genetic Variation
Humans
Integrin alpha3
Integrins / genetics*
Introns
Luciferases / genetics
Mice
Promoter Regions, Genetic
Transformation, Genetic
|
| IF |
2.476
|
| 引用数 |
9
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
