論文 - 詳細
| RRC ID | 39365 |
|---|---|
| 著者 | Watabe A, Yamaguchi T, Kawanishi T, Uchida E, Eguchi A, Mizuguchi H, Mayumi T, Nakanishi M, Hayakawa T. |
| タイトル | Target-cell specificity of fusogenic liposomes: membrane fusion-mediated macromolecule delivery into human blood mononuclear cells. |
| ジャーナル | Biochim Biophys Acta |
| Abstract |
Fusogenic liposome, a unique vector prepared by fusing ultraviolet-inactivated Sendai virus and liposome, is known to efficiently deliver content into various animal cells through membrane fusion. In this study, we examined the target-cell specificity of fusogenic liposome (FL)-mediated macromolecule delivery into human blood cells using diphtheria toxin fragment A (DTA) as a probe. Among the peripheral blood mononuclear cells (PBMC), FL was able to deliver its encapsulates into CD14+ monocytes and CD4-/CD8- T-cells, but not into CD19+ B-lymphocytes, CD4+ T-cells or CD8+ T-cells. The susceptibility of human leukemia cell lines to FL was similar to that of PBMC; the order of the reactivity was U937 (monoblastic leukemia)>MOLT4, Jurkat (T-lymphoma)>Daudi, BALL1 (B-lymphoma)>K562 (erythroblastic leukemia). Interestingly, FL showed similar binding activity to all of these leukemia cell lines. These findings indicate that, among blood cells, monocytes, monoblastic leukemia cells, CD4-/CD8- T-cells and T-lymphoma cells are preferable targets for FL-mediated macromolecule delivery. This is the first demonstration of the existence of non-permissive cells against FL. Our results also suggest that some molecules on target-cells other than the binding targets of SV-derived protein may participate in fusion between FL and cells. |
| 巻・号 | 1416(1-2) |
| ページ | 339-48 |
| 公開日 | 1999-1-12 |
| DOI | 10.1016/s0005-2736(98)00238-7 |
| PII | S0005-2736(98)00238-7 |
| PMID | 9889393 |
| MeSH | Diphtheria Toxin / pharmacology Drug Carriers HeLa Cells Humans Intracellular Membranes / physiology* Liposomes Membrane Fusion* Monocytes / physiology* Monocytes / ultrastructure Peptide Fragments / pharmacology Respirovirus / physiology T-Lymphocytes Tumor Cells, Cultured Viral Proteins / physiology |
| IF | 3.411 |
| 引用数 | 11 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Jurkat(RCB0806) |