| RRC ID |
39368
|
| 著者 |
Kukita A, Kukita T, Ouchida M, Maeda H, Yatsuki H, Kohashi O.
|
| タイトル |
Osteoclast-derived zinc finger (OCZF) protein with POZ domain, a possible transcriptional repressor, is involved in osteoclastogenesis.
|
| ジャーナル |
Blood
|
| Abstract |
The differentiation of osteoclasts is regulated by transcription factors expressed in cells of osteoclast lineage. We isolated here a potential transcription factor from a cDNA library of an enriched population of preosteoclasts and osteoclasts. The cDNA encodes a protein with N-terminal POZ domain and C-terminal Krüppel-like zinc fingers. We designate this protein as osteoclast-derived zinc finger (OCZF). OCZF was found to be rat homologue of mouse leukemia/lymphoma-related factor (LRF). Northern blot and in situ hybridization analysis showed OCZF mRNA at a high level in osteoclasts and kidney cells. OCZF had a nuclear targeting sequence and was localized in the nucleus of transfected cells. In addition, OCZF specifically bound to the guanine-rich consensus sequences of Egr-1 and c-Krox. Transient transfection assays indicate that OCZF can repress transcription activity like other POZ domain proteins. Furthermore, antisense but not sense phosphorothioate oligodeoxynucleotides (ODNs) for OCZF cDNA suppressed the formation of osteoclast-like multinucleated cells (MNCs) in bone marrow culture, whereas the same ODNs did not significantly affect the formation of macrophage polykaryons and mononuclear preosteoclast-like cells (POCs). These results suggest that OCZF is a unique transcription factor that plays an important role in the late stage of osteoclastogenesis.
|
| 巻・号 |
94(6)
|
| ページ |
1987-97
|
| 公開日 |
1999-9-15
|
| PII |
S0006-4971(20)48906-2
|
| PMID |
10477728
|
| MeSH |
Amino Acid Sequence
Animals
Cell Line
DNA-Binding Proteins / chemistry
DNA-Binding Proteins / genetics
Humans
Male
Mice
Molecular Sequence Data
Osteoblasts / cytology
Osteoclasts / cytology*
Osteoclasts / metabolism*
Rats
Rats, Sprague-Dawley
Repressor Proteins*
Sequence Alignment
Sequence Homology, Amino Acid
Transcription Factors / chemistry
Transcription Factors / genetics*
Transcription Factors / metabolism*
Transcription, Genetic
Tumor Cells, Cultured
Zinc Fingers
|
| IF |
17.794
|
| 引用数 |
71
|
|
WOS 分野
|
HEMATOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
NRK(RCB0043) |
