RRC ID 39369
著者 Zhao X, Wakamatsu Y, Shibahara M, Nomura N, Geltinger C, Nakahara T, Murata T, Yokoyama KK.
タイトル Mannosylerythritol lipid is a potent inducer of apoptosis and differentiation of mouse melanoma cells in culture.
ジャーナル Cancer Res
Abstract Malignant melanomas are tumors that are well known to respond poorly to treatment with chemotherapeutic reagents. We report here that mannosylerythritol lipid (MEL), an extracellular glycolipid from yeast, markedly inhibited the growth of mouse melanoma B16 cells in a dose-dependent manner. Exposure of B16 cells to MEL at 10 microM and higher concentrations caused the condensation of chromatin, DNA fragmentation, and sub-G1 arrest, all of which are hallmarks of cells that are undergoing apoptosis. Analysis of the cell cycle also suggested that both the MEL-mediated inhibition of growth and apoptosis were closely associated with growth arrest in the G1 phase. Moreover, MEL exposure stimulated the expression of differentiation markers of melanoma cells, such as tyrosinase activity and the enhanced production of melanin, which is an indication that MEL triggered both apoptotic and cell differentiation programs. Forced expression of Bcl-2 protein in stably transformed B16 cells had a dual effect: it interfered with MEL-induced apoptosis but increased both tyrosinase activity and the production of melanin as compared with these phenomena in vector-transfected MEL-treated control B16 cells. These results provide the first evidence that growth arrest, apoptosis, and the differentiation of mouse malignant melanoma cells can be induced by a microbial extracellular glycolipid.
巻・号 59(2)
ページ 482-6
公開日 1999-1-15
PMID 9927066
MeSH Animals Apoptosis / drug effects* Candida / chemistry Cell Cycle / drug effects Cell Differentiation / drug effects Dose-Response Relationship, Drug Glycolipids / pharmacology* Melanoma, Experimental / pathology* Mice Protein Kinase C / physiology Proto-Oncogene Proteins c-bcl-2 / analysis
IF 9.727
引用数 72
WOS 分野 ONCOLOGY
リソース情報
ヒト・動物細胞 B16 melanoma 4A5(RCB0557)