Reference - Detail
| RRC ID | 39378 |
|---|---|
| Author | Fujii M, Takeda K, Imamura T, Aoki H, Sampath TK, Enomoto S, Kawabata M, Kato M, Ichijo H, Miyazono K. |
| Title | Roles of bone morphogenetic protein type I receptors and Smad proteins in osteoblast and chondroblast differentiation. |
| Journal | Mol Biol Cell |
| Abstract |
The biological effects of type I serine/threonine kinase receptors and Smad proteins were examined using an adenovirus-based vector system. Constitutively active forms of bone morphogenetic protein (BMP) type I receptors (BMPR-IA and BMPR-IB; BMPR-I group) and those of activin receptor-like kinase (ALK)-1 and ALK-2 (ALK-1 group) induced alkaline phosphatase activity in C2C12 cells. Receptor-regulated Smads (R-Smads) that act in the BMP pathways, such as Smad1 and Smad5, also induced the alkaline phosphatase activity in C2C12 cells. BMP-6 dramatically enhanced alkaline phosphatase activity induced by Smad1 or Smad5, probably because of the nuclear translocation of R-Smads triggered by the ligand. Inhibitory Smads, i.e., Smad6 and Smad7, repressed the alkaline phosphatase activity induced by BMP-6 or the type I receptors. Chondrogenic differentiation of ATDC5 cells was induced by the receptors of the BMPR-I group but not by those of the ALK-1 group. However, kinase-inactive forms of the receptors of the ALK-1 and BMPR-I groups blocked chondrogenic differentiation. Although R-Smads failed to induce cartilage nodule formation, inhibitory Smads blocked it. Osteoblast differentiation induced by BMPs is thus mediated mainly via the Smad-signaling pathway, whereas chondrogenic differentiation may be transmitted by Smad-dependent and independent pathways. |
| Volume | 10(11) |
| Pages | 3801-13 |
| Published | 1999-11-1 |
| DOI | 10.1091/mbc.10.11.3801 |
| PMID | 10564272 |
| PMC | PMC25680 |
| MeSH | Activin Receptors Adenoviridae / genetics Alkaline Phosphatase / metabolism Animals Bone Morphogenetic Protein Receptors, Type I Cell Differentiation Cell Line Chondrocytes DNA-Binding Proteins / metabolism* Genetic Vectors Histocytochemistry Mice Osteoblasts Phosphoproteins / metabolism Phosphorylation Protein Serine-Threonine Kinases / metabolism* Receptors, Growth Factor / metabolism* Smad Proteins Smad1 Protein Smad5 Protein Smad6 Protein Smad7 Protein Trans-Activators / metabolism* |
| IF | 3.791 |
| Times Cited | 339 |
| WOS Category | CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 22 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | ATDC5(RCB0565) |