RRC ID 39402
Author Kishikawa T, Ghazizadeh M, Sasaki Y, Springer GF.
Title Specific role of T and Tn tumor-associated antigens in adhesion between a human breast carcinoma cell line and a normal human breast epithelial cell line.
Journal Jpn. J. Cancer Res.
Abstract The possibility that tumor-associated antigens T and Tn act as adhesion molecules between normal and malignant breast epithelial cells at the early stages of recognition in the metastatic pathway was examined in vitro. The adhesive specificity of the antigens was assessed by means of in vitro adhesion tests between a carcinomatous breast cancer cell line (ZR75-30) and a normal epithelial breast cell line (HLB100) using both monoclonal antibodies and lectins specific as well as nonspecific for each antigen. Adhesion assay was performed using monolayers of the normal cell line prepared on plastic culture plates and the tumor cell line labeled with a fluorescent dye as a probe. The adhesion between the two cell types occurred with significant specificity via T and Tn antigens (P<0.001), and was temperature-dependent. The results suggest that at the early stages of recognition by tumor cells in the metastatic process, T and Tn antigens play a role as adhesion molecules between the tumor cells and adjacent normal cells.
Volume 90(3)
Pages 326-32
Published 1999-3
DOI 10.1111/j.1349-7006.1999.tb00751.x
PII S0910505099800994
PMID 10359048
PMC PMC5926062
MeSH Antibodies, Monoclonal / immunology Antigens, Neoplasm / immunology Antigens, Neoplasm / physiology* Antigens, Tumor-Associated, Carbohydrate / immunology Antigens, Tumor-Associated, Carbohydrate / physiology* Breast / cytology* Breast / drug effects Breast / metabolism Breast / pathology Breast Neoplasms / metabolism Breast Neoplasms / pathology* Cell Adhesion / drug effects Cell Adhesion Molecules / immunology Cell Adhesion Molecules / metabolism Cell Aggregation / drug effects Cell Line Coculture Techniques Epithelial Cells / cytology* Epithelial Cells / drug effects Epithelial Cells / metabolism Female Fluorescent Dyes Humans Immunohistochemistry Kinetics Neoplasm Metastasis Peanut Agglutinin / pharmacology Temperature Tumor Cells, Cultured
Times Cited 13
Human and Animal Cells