RRC ID 39406
Author Joki T, Kikuchi T, Akasaki Y, Saitoh S, Abe T, Ohno T.
Title Induction of effective antitumor immunity in a mouse brain tumor model using B7-1 (CD80) and intercellular adhesive molecule 1 (ICAM-1; CD54) transfection and recombinant interleukin 12.
Journal Int J Cancer
Abstract Although tumor-specific T lymphocytes recognize tumor-associated antigens (TAA) present on their cell surface via major histocompatibility complex (MHC) molecules, T cells require other activating signals. These are provided by costimulatory molecules, including B7-1 (CD80), B7-2 (CD86) and intercellular adhesive molecule 1 (ICAM-1; CD54). Transfecting mouse tumor cell lines with the B7 gene can lead to primary tumor rejection and the establishment of protective immunity. However, some studies have shown that the B7 effect upon T-cell-dependent tumor immunity is limited. Therefore, we examined the antitumor effects of recombinant interleukin 12 (IL-12) and genetically engineered glioma cells expressing B7-1 or both B7-1 and ICAM-1. Vaccination of mice with B7-1-expressing tumor cells substantially inhibited the growth of subcutaneously inoculated gliomas but not those located in the brain. Vaccination with B7-1-expressing tumor cells and systemic recombinant IL-12 (rIL-12) was more effective than either B7-1-expressing tumor cells or rIL-12 alone. Our murine brain tumor model also showed that vaccination with tumor cells expressing both B7-1 and ICAM-1 combined with rIL-12 prolonged survival. We have demonstrated the therapeutic potential of vaccination with rIL-12 and tumor cells expressing both B7-1 and ICAM-1 in the control of glioma growth.
Volume 82(5)
Pages 714-20
Published 1999-8-27
DOI 10.1002/(sici)1097-0215(19990827)82:5<714::aid-ijc15>3.0.co;2-q
PII 10.1002/(SICI)1097-0215(19990827)82:5<714::AID-IJC15>3.0.CO;2-Q
PMID 10417770
MeSH Animals Antibodies, Neoplasm / therapeutic use B7-1 Antigen / genetics B7-1 Antigen / immunology* Brain Neoplasms / immunology Brain Neoplasms / mortality Brain Neoplasms / therapy* CD8-Positive T-Lymphocytes / immunology Disease Models, Animal Female Glioma / genetics Glioma / pathology Humans Immunotherapy, Active Intercellular Adhesion Molecule-1 / genetics Intercellular Adhesion Molecule-1 / immunology* Interleukin-12 / immunology* Mice Mice, Nude Mice, SCID Neoplasm Transplantation T-Lymphocyte Subsets / immunology T-Lymphocytes / physiology T-Lymphocytes, Cytotoxic / immunology Transfection Tumor Cells, Cultured
IF 4.982
Times Cited 38
Human and Animal Cells