RRC ID 3942
Author Purta E, van Vliet F, Tkaczuk KL, Dunin-Horkawicz S, Mori H, Droogmans L, Bujnicki JM.
Title The yfhQ gene of Escherichia coli encodes a tRNA:Cm32/Um32 methyltransferase.
Journal BMC Mol Biol
Abstract BACKGROUND:Naturally occurring tRNAs contain numerous modified nucleosides. They are formed by enzymatic modification of the primary transcripts during the complex RNA maturation process. In model organisms Escherichia coli and Saccharomyces cerevisiae most enzymes involved in this process have been identified. Interestingly, it was found that tRNA methylation, one of the most common modifications, can be introduced by S-adenosyl-L-methionine (AdoMet)-dependent methyltransferases (MTases) that belong to two structurally and phylogenetically unrelated protein superfamilies: RFM and SPOUT.
RESULTS:As a part of a large-scale project aiming at characterization of a complete set of RNA modification enzymes of model organisms, we have studied the Escherichia coli proteins YibK, LasT, YfhQ, and YbeA for their ability to introduce the last unassigned methylations of ribose at positions 32 and 34 of the tRNA anticodon loop. We found that YfhQ catalyzes the AdoMet-dependent formation of Cm32 or Um32 in tRNASer1 and tRNAGln2 and that an E. coli strain with a disrupted yfhQ gene lacks the tRNA:Cm32/Um32 methyltransferase activity. Thus, we propose to rename YfhQ as TrMet(Xm32) according to the recently proposed, uniform nomenclature for all RNA modification enzymes, or TrmJ, according to the traditional nomenclature for bacterial tRNA MTases.
CONCLUSION:Our results reveal that methylation at position 32 is carried out by completely unrelated TrMet(Xm32) enzymes in eukaryota and prokaryota (RFM superfamily member Trm7 and SPOUT superfamily member TrmJ, respectively), mirroring the scenario observed in the case of the m1G37 modification (introduced by the RFM member Trm5 in eukaryota and archaea, and by the SPOUT member TrmD in bacteria).
Volume 7
Pages 23
Published 2006-7-18
DOI 10.1186/1471-2199-7-23
PII 1471-2199-7-23
PMID 16848900
PMC PMC1569432
MeSH Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism Escherichia coli Proteins / physiology* Eukaryotic Cells Methylation Prokaryotic Cells RNA, Transfer / metabolism Terminology as Topic tRNA Methyltransferases / genetics tRNA Methyltransferases / metabolism tRNA Methyltransferases / physiology*
IF 2.568
Times Cited 34
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Prokaryotes E. coli Keno? ME9042(BL21(DE3))