| RRC ID |
39426
|
| 著者 |
Goseki-Sone M, Yamada A, Asahi K, Hirota A, Ezawa I, Iimura T.
|
| タイトル |
Phosphate depletion enhances tissue-nonspecific alkaline phosphatase gene expression in a cultured mouse marrow stromal cell line ST2.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Alkaline phosphatases (ALP) are highly ubiquitous enzymes present in the majority of animals from bacteria to higher vertebrate. Although their wide distribution in nature has suggested that these enzymes should perform important biological functions, their detailed roles or natural substrates remain unknown. In Escherichia coli, the extracellular phosphate (Pi) limitation induces the ALP gene, indicating the role of extracellular Pi in ALP gene regulation. However, little is known about the similar mechanisms in mammalian cells. This study was designed to examine the effect of low Pi medium on the ALP activity and its expression in the mouse stromal cell line ST2. The enzymatic property was classified into tissue-nonspecific ALP (TNSALP). After treatment by Pi starvation for 3 days, there was a 2-fold increase in the specific activity of TNSALP. RT-PCR analysis revealed that the mRNA of the TNSALP gene was highly stimulated. These results indicated that the effect of Pi depletion on ALP activity was regulated at the TNSALP transcriptional level, suggesting that the possible role of the Pi sensing system for biological functions of ALP might have been conserved in evolution. Our findings also made it possible to discuss the physiological roles of ALP in vivo.
|
| 巻・号 |
265(1)
|
| ページ |
24-8
|
| 公開日 |
1999-11-1
|
| DOI |
10.1006/bbrc.1999.1624
|
| PII |
S0006-291X(99)91624-8
|
| PMID |
10548485
|
| MeSH |
Alkaline Phosphatase / genetics*
Alkaline Phosphatase / metabolism
Animals
Bone Marrow Cells / enzymology*
Cell Line
Gene Expression Regulation, Enzymologic* / drug effects
Kinetics
Mice
Phosphates / metabolism*
Phosphates / pharmacology*
Reverse Transcriptase Polymerase Chain Reaction
Stromal Cells / enzymology*
Time Factors
|
| IF |
2.985
|
| 引用数 |
13
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
ST2(RCB0224) |
