| RRC ID |
39427
|
| Author |
Hosoya Y, Kitoh Y, Kobayashi E, Okabe R, Fujimura A, Kanazawa K.
|
| Title |
Combination effects of tamoxifen plus 5-fluorouracil on gastric cancer cell lines in vitro.
|
| Journal |
Cancer Lett
|
| Abstract |
We tested the effect of tamoxifen alone and tamoxifen plus 5-fluorouracil (5-FU) on proliferation of two different types of gastric cancer cell lines using the WST-1 method. A high dose of tamoxifen suppressed the proliferation of KATOIII cells (poorly differentiated adenocarcinoma), but MKN28 cells (well-differentiated adenocarcinoma) were not affected. The combination of the two drugs resulted in a synergistic anti-proliferative activity on KATOIII cells. On the other hand, in the combination therapy, tamoxifen stimulated MKN28 cells to proliferate in a dose-dependent manner. TGF-beta1 secretion was not changed in KATOIII cells by tamoxifen plus 5-FU treatment but was down-regulated in MKN28 cells. Both cancer cell lines were judged as intracellular estrogen receptor (ER) negative. These data suggest that the anti-proliferative effects of tamoxifen plus 5-FU on KATOIII cells were not dependent on ER expression or TGF-beta1 secretion. On the other hand, their proliferative effects on MKN28 cells might be, in part, caused by the reduced secretion of TGF-beta1.
|
| Volume |
140(1-2)
|
| Pages |
139-43
|
| Published |
1999-6-1
|
| DOI |
10.1016/s0304-3835(99)00059-2
|
| PII |
S0304-3835(99)00059-2
|
| PMID |
10403552
|
| MeSH |
Adenocarcinoma / metabolism
Adenocarcinoma / pathology
Antineoplastic Combined Chemotherapy Protocols / pharmacology*
Carcinoma, Signet Ring Cell / metabolism
Carcinoma, Signet Ring Cell / pathology
Cell Division / drug effects
Dose-Response Relationship, Drug
Fluorouracil / administration & dosage
Fluorouracil / pharmacology
Humans
Receptors, Estrogen / metabolism
Stomach Neoplasms / metabolism
Stomach Neoplasms / pathology*
Tamoxifen / administration & dosage
Tamoxifen / pharmacology
Time Factors
Transforming Growth Factor beta / metabolism
Tumor Cells, Cultured
|
| IF |
7.36
|
| Times Cited |
8
|
|
WOS Category
|
ONCOLOGY
|
| Resource |
| Human and Animal Cells |
MKN28(RCB1000) |
