RRC ID 39443
Author Miyata R, Iwabuchi K, Watanabe S, Sato N, Nagaoka I.
Title Short exposure of intestinal epithelial cells to TNF-alpha and histamine induces Mac-1-mediated neutrophil adhesion independent of protein synthesis.
Journal J Leukoc Biol
Abstract Neutrophils play an important role in intestinal inflammation by interacting with intestinal epithelial cells. In this study, we evaluated neutrophil adhesion to intestinal epithelial cells using intestinal epithelial cell line HT29 stimulated with tumor necrosis factor alpha (TNF-alpha) and histamine for a short time (30 min). The TNF-alpha and histamine stimulation markedly increased neutrophil adhesion. The increased adhesion was inhibited by anti-CD11b and anti-CD18 monoclonal antibodies (mAbs), but not by anti-CD11a and anti-CD54 (ICAM-1) mAbs. It is interesting that flow cytometric analysis revealed that ICAM-1 expression on HT29 cells was not changed by TNF-alpha and histamine stimulation. Moreover, the increased adhesion was inhibited by proteinase K treatment but not cycloheximide treatment of HT29 cells. Together these observations suggest that short exposure of HT29 cells to TNF-alpha and histamine induces CD11b/CD18 (Mac-1)-dependent but CD11a/CD18 (LFA-1)-independent neutrophil adhesion to intestinal epithelial cells, and ICAM-1 is not likely to be involved in the interactions. Furthermore, epithelial cell ligand(s) for neutrophils is likely protein molecule(s) that is expressed on the cell by stimulation independent protein synthesis. However, it is also possible that neutrophil activating factor(s), which stimulates neutrophils to bind with epithelial ligands via Mac-1, is expressed by epithelial cells during stimulation.
Volume 66(3)
Pages 437-46
Published 1999-9-1
DOI 10.1002/jlb.66.3.437
PMID 10496314
MeSH Adenocarcinoma / pathology Antibodies, Monoclonal / pharmacology Antimetabolites / pharmacology CD18 Antigens / physiology Cations, Divalent / pharmacology Cell Adhesion / drug effects Cell Polarity Colonic Neoplasms / pathology Cycloheximide / pharmacology Endopeptidase K / pharmacology Epithelial Cells / drug effects Hexosaminidases / pharmacology Histamine / pharmacology* Humans Intercellular Adhesion Molecule-1 / physiology Intestinal Mucosa / cytology Intestinal Mucosa / drug effects* Lymphocyte Function-Associated Antigen-1 / physiology Macrophage-1 Antigen / physiology* Neoplasm Proteins / physiology Neuraminidase / pharmacology Neutrophils / cytology Neutrophils / drug effects* Phosphatidylinositol Diacylglycerol-Lyase Recombinant Proteins / pharmacology Stimulation, Chemical Tumor Cells, Cultured / drug effects Tumor Cells, Cultured / metabolism Tumor Necrosis Factor-alpha / pharmacology* Tunicamycin / pharmacology Type C Phospholipases / pharmacology
IF 3.757
Times Cited 18
Human and Animal Cells CACO-2(RCB0988)