Reference - Detail
| RRC ID | 39446 |
|---|---|
| Author | Maekawa K, Yoshikawa N, Du J, Nishida S, Kitasato H, Okamoto K, Tanaka H, Mizushima Y, Kawai S. |
| Title | The molecular mechanism of inhibition of interleukin-1beta-induced cyclooxygenase-2 expression in human synovial cells by Tripterygium wilfordii Hook F extract. |
| Journal | Inflamm Res |
| Abstract |
OBJECTIVE:Several extracts of Tripterygium wilfordii Hook F (TWHF) have been reported to be effective in patients with rheumatoid arthritis. We investigated the effect of multi-glycosides ofTWHF (GTW), a TWHF extract, on interleukin (IL)-1beta stimulated human rheumatoid synovial cells. MATERIALS AND METHODS:IL-1beta-stimulated synovial cells were used to detect the effects of GTW on cyclooxygenase (COX)-1 and COX-2 activities, expression of COX protein and mRNA, and nuclear transcription factors in experiments using respective reporter plasmids. RESULTS:GTW inhibited prostaglandin E2 production by IL-1beta-stimulated synovial cells in a concentration-dependent manner, and also inhibited COX-2 protein and mRNA expression in a similar fashion to dexamethasone. However, GTW did not act as a glucocorticoid agonist. GTW repressed IL-1beta-induced nuclear factor-kappaB activity, but did not have a significant influence on activating protein-1 activity. CONCLUSION:The anti-rheumatic effect of GTW or TWHF may be partly mediated through the inhibition of prostaglandin E2 production in human synovial cells due to suppression of COX-2 mRNA, possibly via inhibition of nuclear factor-kappaB activity. |
| Volume | 48(11) |
| Pages | 575-81 |
| Published | 1999-11-1 |
| DOI | 10.1007/s000110050506 |
| PMID | 10598013 |
| MeSH | Animals Anti-Inflammatory Agents / pharmacology Arthritis, Rheumatoid / metabolism Blotting, Northern Blotting, Western COS Cells Cyclooxygenase 2 Cyclooxygenase 2 Inhibitors Cyclooxygenase Inhibitors / pharmacology* Dexamethasone / pharmacology Dinoprostone / biosynthesis Gene Expression Regulation / drug effects Genes, Reporter / genetics Humans Interleukin-1 / antagonists & inhibitors* Interleukin-1 / pharmacology Isoenzymes / biosynthesis* Luciferases / biosynthesis Luciferases / genetics Membrane Proteins Plant Extracts / pharmacology* Plants, Medicinal / chemistry* Prostaglandin-Endoperoxide Synthases / biosynthesis* RNA, Messenger / biosynthesis Synovial Membrane / cytology Synovial Membrane / drug effects Synovial Membrane / metabolism* Transcription Factors / biosynthesis |
| IF | 3.174 |
| Times Cited | 21 |
| WOS Category | IMMUNOLOGY CELL BIOLOGY |
| Resource | |
| Human and Animal Cells | COS-7(RCB0539) |