RRC ID 39451
Author Kawakami K, Qureshi MH, Koguchi Y, Nakajima K, Saito A.
Title Differential effect of Cryptococcus neoformans on the production of IL-12p40 and IL-10 by murine macrophages stimulated with lipopolysaccharide and gamma interferon.
Journal FEMS Microbiol. Lett.
Abstract In the present study, we examined the in vitro effect of Cryptococcus neoformans on the production of interleukin-12 (IL-12) and IL-10 by murine macrophages. At a dose of 1 x 10(5), 1 x 10(6) or 1 x 10(7) ml-1, a highly virulent strain of C. neoformans (strain YC-11) suppressed the production of IL-12p40 by a murine macrophage cell line, J774.1 stimulated with lipopolysaccharide (LPS) and interferon (IFN)-gamma, while the production of IL-10 was not inhibited, but rather slightly augmented. The suppression of IL-12p40 production did not change by neutralizing anti-IL-10 mAb. A direct contact of C. neoformans with macrophages was largely involved in this inhibitory effect, since placement of a 0.45 micron pore membrane between the organism and macrophages prevented such effect. On the other hand, the culture supernatant of YC-11 did not inhibit macrophage IL-12p40 production when used at a lower dose, which contained an equivalent amount of capsular polysaccharide to that in the supernatant of YC-11 cultured at 1 x 10(5) or 1 x 10(6) ml-1, although it showed a small suppression at higher doses. Our results suggest that C. neoformans may suppress the induction of Th1 responses by inhibiting macrophage IL-12 production predominantly through a direct contact-dependent mechanism and to a lesser extent by a certain soluble factor(s) released from this microorganism.
Volume 175(1)
Pages 87-94
Published 1999-6-1
DOI 10.1111/j.1574-6968.1999.tb13605.x
PII S0378-1097(99)00172-X
PMID 10361712
MeSH Animals Cell Line Cryptococcosis / microbiology Cryptococcus neoformans / growth & development Cryptococcus neoformans / immunology* Cryptococcus neoformans / isolation & purification Humans Interferon-gamma / pharmacology Interleukin-10 / biosynthesis* Interleukin-12 / biosynthesis* Lipopolysaccharides / pharmacology Lung Diseases, Fungal / microbiology Macrophage Activation Macrophages / drug effects Macrophages / immunology* Macrophages / microbiology* Mice
IF 1.735
Times Cited 16
Human and Animal Cells