| RRC ID |
39454
|
| Author |
Ohta S, Muramatsu H, Senda T, Zou K, Iwata H, Muramatsu T.
|
| Title |
Midkine is expressed during repair of bone fracture and promotes chondrogenesis.
|
| Journal |
J Bone Miner Res
|
| Abstract |
Midkine (MK) is a heparin-binding growth/differentiation factor implicated in the control of development and repair of various tissues. Upon fracture of the murine tibia, MK was found to be transiently expressed during bone repair. MK was immunohistochemically detected in spindle-shaped mesenchymal cells at the fracture site on day 4 after fracture and in chondrocytes in the area of endochondral ossification on day 7. MK expression was decreased on day 14 and scarcely seen on day 28 when bone repair was completed. This mode of MK expression is reminiscent of MK expression during development. MK was expressed in hypertrophic chondrocytes of the prebone cartilage rudiments on embryonic day 14 in mouse embryos. MK was also strongly expressed in the epiphyseal growth plate. MK was localized intracellularly during both bone repair and development, and this localization was confirmed by immunoelectron microscopy for embryonic chondrocytes. When MK cDNA was transfected into ATDC5 chondrogenic cells and overexpressed, the majority of transfected cells with strong MK expression showed enhanced chondrogenesis as revealed by increased synthesis of sulfated glycosaminoglycans, aggrecan, and type II collagen. These results suggest that MK plays important roles in chondrogenesis and contributes to bone formation and repair.
|
| Volume |
14(7)
|
| Pages |
1132-44
|
| Published |
1999-7-1
|
| DOI |
10.1359/jbmr.1999.14.7.1132
|
| PII |
jbm297
|
| PMID |
10404013
|
| MeSH |
Aggrecans
Animals
Animals, Newborn
Blotting, Northern
Carrier Proteins / biosynthesis*
Carrier Proteins / genetics
Cell Line
Chondrocytes / metabolism
Chondrocytes / ultrastructure
Chondrogenesis / genetics
Chondrogenesis / physiology*
Cytokines / biosynthesis
Cytokines / genetics
DNA, Complementary / genetics
Embryo, Mammalian
Extracellular Matrix Proteins*
Fracture Healing / physiology*
Growth Plate / cytology
Growth Plate / metabolism
Immunohistochemistry
Lectins, C-Type
Limb Buds / metabolism
Limb Buds / ultrastructure
Mice
Mice, Inbred ICR
Microscopy, Immunoelectron
Midkine
Osteogenesis / genetics
Osteogenesis / physiology*
Proteoglycans / biosynthesis
Transfection
|
| IF |
5.854
|
| Times Cited |
47
|
|
WOS Category
|
ENDOCRINOLOGY & METABOLISM
|
| Resource |
| Human and Animal Cells |
ATDC5(RCB0565) |
