論文 - 詳細
| RRC ID | 39474 |
|---|---|
| 著者 | Kimura A, Yoshida I, Takagi N, Takahashi T. |
| タイトル | Structure and localization of the mouse prolyl oligopeptidase gene. |
| ジャーナル | J Biol Chem |
| Abstract |
We have cloned and characterized the genomic structure of the mouse gene for prolyl oligopeptidase that is mapped to chromosome 10B2-B3. The gene is about 92 kilobases in size and contains 15 exons. All exon-intron junction sequences conform to the GT/AG rule. Comparison with the presumed domain structures of the mouse prolyl oligopeptidase indicates that the propeller domain of the enzyme is encoded by exons 3-10, whereas the catalytic domain is encoded by exons 1-3 and 10-15. The catalytic triad residues are encoded by two exons (Ser(554) on exon 13 and His(680) and Asp(642) on exon 15). The 5'-flanking region of the mouse prolyl oligopeptidase gene has structural features found in housekeeping gene promoters, including a GC-rich segment and an absence of TATA and CAAT boxes. A primer extension assay showed the presence of multiple sites for the initiation of transcription. Transient transfection analysis demonstrated that the 5'-flanking region of the gene can direct efficient expression in COS1 cells. Deletion studies revealed that the downstream 125-base pair sequence of the region is required for promoter activity in the cells. |
| 巻・号 | 274(34) |
| ページ | 24047-53 |
| 公開日 | 1999-8-20 |
| DOI | 10.1074/jbc.274.34.24047 |
| PII | S0021-9258(19)55527-2 |
| PMID | 10446174 |
| MeSH | Animals Base Sequence Catalytic Domain Chromosome Mapping Cloning, Molecular Dipeptidyl Peptidase 4 / chemistry Dipeptidyl Peptidase 4 / genetics Female Mice Mice, Inbred C57BL Molecular Sequence Data Prolyl Oligopeptidases Promoter Regions, Genetic Serine Endopeptidases / chemistry Serine Endopeptidases / genetics* TATA Box |
| IF | 4.238 |
| 引用数 | 20 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | COS-1(RCB0143) |