論文 - 詳細
| RRC ID | 3948 |
|---|---|
| 著者 | Garza-Sánchez F, Gin JG, Hayes CS. |
| タイトル | Amino acid starvation and colicin D treatment induce A-site mRNA cleavage in Escherichia coli. |
| ジャーナル | J Mol Biol |
| Abstract |
Escherichia coli possesses a unique RNase activity that cleaves stop codons in the ribosomal aminoacyl-tRNA binding site (A-site) during inefficient translation termination. This A-site mRNA cleavage allows recycling of arrested ribosomes by facilitating recruitment of the tmRNA*SmpB ribosome rescue system. To test whether A-site nuclease activity also cleaves sense codons, we induced ribosome pausing at each of the six arginine codons using three strategies; rare codon usage, arginine starvation, and inactivation of arginine tRNAs with colicin D. In each instance, ribosome pausing induced mRNA cleavage within the target arginine codons, and resulted in tmRNA-mediated SsrA-peptide tagging of the nascent polypeptide. A-site mRNA cleavage did not require the stringent factor ppGpp, or bacterial toxins such as RelE, which mediates a similar nuclease activity. However, the efficiency of A-site cleavage was modulated by the identity of the two codons immediately upstream (5' side) of the A-site codon. Starvation for histidine and tryptophan also induced A-site cleavage at histidine and tryptophan codons, respectively. Thus, A-site mRNA cleavage is a general response to ribosome pausing, capable of cleaving a variety of sense and stop codons. The induction of A-site cleavage during amino acid starvation suggests this nuclease activity may help to regulate protein synthesis during nutritional stress. |
| 巻・号 | 378(3) |
| ページ | 505-19 |
| 公開日 | 2008-5-2 |
| DOI | 10.1016/j.jmb.2008.02.065 |
| PII | S0022-2836(08)00275-1 |
| PMID | 18377929 |
| PMC | PMC2409371 |
| MeSH | Amino Acids / metabolism* Binding Sites Codon, Terminator Escherichia coli / genetics Escherichia coli / metabolism* Escherichia coli Proteins / metabolism* Protein Biosynthesis RNA, Messenger / metabolism* RNA, Transfer / metabolism |
| IF | 4.76 |
| 引用数 | 41 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 原核生物(大腸菌) | Keio collection |