Reference - Detail
RRC ID | 39485 |
---|---|
Author | Yoshida A, Minowa MT, Takamatsu S, Hara T, Oguri S, Ikenaga H, Takeuchi M. |
Title | Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase. |
Journal | Glycobiology |
Abstract |
A human cDNA for UDP- N -acetylglucosamine:alpha1,3-d-mannoside beta1,4- N- acetylglucosaminyltransferase (GnT-IV) was isolated from a liver cDNA library using a probe based on a partial cDNA sequence of the bovine GnT-IV. The cDNA encoded a complete sequence of a type II membrane protein of 535 amino acids which is 96% identical to the bovine GnT-IV. Transient expression of the human cDNA in COS7 cells increased total cellular GnT-IV activity 25-fold, demonstrating that this cDNA encodes a functional human GnT-IV. Northern blot analysis of normal tissues indicated that at least five different sizes of mRNA (9.7, 7.6, 5.1, 3.8, and 2.4 kb) forGnT-IV are expressed in vivo. Furthermore, these mRNAs are expressed at different levels between tissues. Large amounts of mRNA were detected in tissues harboring T lineage cells. Also, the promyelocytic leukemia cell line HL-60 and the lymphoblastic leukemia cell line MOLT-4 revealed abundant mRNA. Lastly, the gene was mapped at the locus on human chromosome 2, band q12 by fluorescent in situ hybridization. |
Volume | 9(3) |
Pages | 303-10 |
Published | 1999-3-1 |
DOI | 10.1093/glycob/9.3.303 |
PII | cwc029 |
PMID | 10024668 |
MeSH | Amino Acid Sequence Animals Base Sequence Blotting, Northern COS Cells Cell Lineage Chromosome Mapping Chromosomes, Human, Pair 2 / genetics* DNA, Complementary / genetics Gene Expression Humans In Situ Hybridization, Fluorescence Leukemia / enzymology Lymphoid Tissue / enzymology Membrane Proteins / genetics* Molecular Sequence Data N-Acetylglucosaminyltransferases / genetics* Neoplasm Proteins / genetics RNA, Messenger / isolation & purification Recombinant Proteins / biosynthesis T-Lymphocytes / enzymology* Tissue Distribution Tumor Cells, Cultured |
IF | 4.06 |
Times Cited | 21 |
WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
Resource | |
Human and Animal Cells |