論文 - 詳細
| RRC ID | 39489 |
|---|---|
| 著者 | Nanbu-Wakao R, Fujitani Y, Masuho Y, Muramatu M, Wakao H. |
| タイトル | Prolactin enhances CCAAT enhancer-binding protein-beta (C/EBP beta) and peroxisome proliferator-activated receptor gamma (PPAR gamma) messenger RNA expression and stimulates adipogenic conversion of NIH-3T3 cells. |
| ジャーナル | Mol Endocrinol |
| Abstract |
Extracellular stimuli trigger adipocyte differentiation by inducing the complex cascades of transcription. Transcription factors CCAAT enhancer-binding proteins (C/EBPs) and peroxisome proliferator-activated receptor gamma (PPARgamma) play crucial roles in this process. Although ectopic expression of these factors in NIH-3T3 cells, a multipotential mesenchymal stem cell line, results in adipogenic conversion, little is known as to hormonal factors that regulate adipogenesis in these cells. In this report we demonstrate that PRL, a lactogenic hormone, enhances C/EBPbeta and PPARbeta mRNA expression and augments adipogenic conversion of NIH-3T3 cells. Moreover, we show that ectopic expression of the PRL receptor in NIH-3T3 cells results in efficient adipocyte conversion when stimulated with PRL and a PPARgamma ligand, as evidenced by expression of the adipocyte differentiation-specific genes as well as the presence of fat-laden cells. We further demonstrate that signal transducer and activator of transcription 5 (Stat5), a PRL signal transducer, activates aP2 promoter in a PRL-dependent manner. These results suggest that PRL acts as an adipogenesis-enhancing hormone in NIH-3T3 cells. |
| 巻・号 | 14(2) |
| ページ | 307-16 |
| 公開日 | 2000-2-1 |
| DOI | 10.1210/mend.14.2.0420 |
| PMID | 10674402 |
| MeSH | 3T3 Cells / drug effects 3T3 Cells / pathology Adipocytes / drug effects Adipocytes / metabolism Animals CCAAT-Enhancer-Binding Proteins DNA-Binding Proteins / drug effects DNA-Binding Proteins / genetics* DNA-Binding Proteins / metabolism Dexamethasone / pharmacology Gene Expression Regulation Mice Milk Proteins* Nuclear Proteins / drug effects Nuclear Proteins / genetics* Nuclear Proteins / metabolism Prolactin / metabolism* Prolactin / pharmacology RNA, Messenger Receptors, Cytoplasmic and Nuclear / drug effects Receptors, Cytoplasmic and Nuclear / genetics* Receptors, Cytoplasmic and Nuclear / metabolism Receptors, Prolactin / metabolism STAT5 Transcription Factor Trans-Activators / drug effects Trans-Activators / metabolism Transcription Factor AP-2 Transcription Factors / drug effects Transcription Factors / genetics* Transcription Factors / metabolism Transcription, Genetic Xanthines / pharmacology |
| IF | 3.628 |
| 引用数 | 71 |
| WOS 分野 | ENDOCRINOLOGY & METABOLISM |
| リソース情報 | |
| ヒト・動物細胞 | NIH-3T3 |