| RRC ID |
39494
|
| Author |
Okada S, Yoshida T, Hong Z, Ishii G, Hatano M, Kuro-O M, Nabeshima Y, Nabeshima Y, Tokuhisa T.
|
| Title |
Impairment of B lymphopoiesis in precocious aging (klotho) mice.
|
| Journal |
Int Immunol
|
| Abstract |
Inactivation of the klotho gene in mice results in multiple disorders that resemble human aging after 3 weeks of age. Because hematopoiesis, especially B lymphopoiesis, is affected in humans and mice by aging, we analyzed the hematopoietic state in homozygous klotho (kl/kl) mice. The kl/kl mice showed thymic atrophy and a reduced number of splenocytes. These mice had almost the normal number of myeloid cells, erythroid cells, IL-3-responsive myeloid precursors and colony forming units in spleen (CFU-S) in bone marrow (BM), but had a substantially decreased number of B cells in BM and peripheral blood as compared with wild-type mice. IL-7-responsive B cell precursors and all of the maturation stages of B cells in BM were also reduced. However, the function of hematopoietic stem cells including their capacity of B lymphopoiesis in vivo and in vitro was normal. Early B cell development was also normal in neonates and young kl/kl mice until 2 weeks old without aging phenotypes. RT-PCR analysis revealed that the level of IL-7 gene expression was significantly reduced in freshly isolated kl/kl BM cells. However, injection of IL-7 in kl/kl mice could not rescue the B lymphopenia. These findings indicate that Klotho protein may regulate B lymphopoiesis via its influence on the hematopoietic microenvironment.
|
| Volume |
12(6)
|
| Pages |
861-71
|
| Published |
2000-6-1
|
| DOI |
10.1093/intimm/12.6.861
|
| PMID |
10837414
|
| MeSH |
Aging / immunology*
Animals
B-Lymphocytes / physiology*
Bone Marrow Cells / physiology
Hematopoiesis*
Hematopoietic Stem Cells / physiology
Interleukin-7 / biosynthesis
Mice
Mice, Inbred C3H
Mice, Mutant Strains
|
| IF |
3.519
|
| Times Cited |
42
|
|
WOS Category
|
IMMUNOLOGY
|
| Resource |
| Human and Animal Cells |
OP9(RCB1124) |
