論文 - 詳細
| RRC ID | 39496 |
|---|---|
| 著者 | Otsuka E, Kato Y, Hirose S, Hagiwara H. |
| タイトル | Role of ascorbic acid in the osteoclast formation: induction of osteoclast differentiation factor with formation of the extracellular collagen matrix. |
| ジャーナル | Endocrinology |
| Abstract |
Osteoclasts are bone-resorbing multinucleated cells. Tartrate-resistant acid phosphatase-positive (TRAP-positive) mononuclear and multinucleated cells, which are osteoclast-like cells (OCLs), were formed as a result of the coculture of mouse bone marrow cells and clonal stromal ST2 cells in the presence of 1alpha,25-dihydroxy-vitamin D3. Removal of ascorbic acid from the culture medium prevented the formation of TRAP-positive OCLs. Addition of ascorbic acid to the medium formed TRAP-positive OCLs, and the effect of ascorbic acid was dose-dependent. When we examined the level of messenger RNA (mRNA) for osteoclast differentiation factor (RANKL/ODF) in ST2 cells, we found that ascorbic acid caused an approximately 5-fold increase in the level of this mRNA. The half-life of the mRNA was unaffected by ascorbic acid. To characterize the mechanism of action of ascorbic acid, we investigated the relationship between formation of TRAP-positive OCLs and formation of the collagen matrix. Inhibitors of the formation of collagen triple helices blocked both the formation of TRAP-positive OCLs and the expression of the mRNA for RANKL/ODF in response to ascorbic acid. Our findings suggest that ascorbic acid might be essential for osteoclastogenesis and might induce the formation of TRAP-positive OCLs via induction of the synthesis of RANKL/ODF that is somehow mediated by the extracellular matrix. |
| 巻・号 | 141(8) |
| ページ | 3006-11 |
| 公開日 | 2000-8-1 |
| DOI | 10.1210/endo.141.8.7597 |
| PMID | 10919289 |
| MeSH | Animals Ascorbic Acid / pharmacology Ascorbic Acid / physiology* Bone Marrow Cells / cytology Bone Marrow Cells / metabolism Calcitriol / pharmacology Carrier Proteins / biosynthesis* Carrier Proteins / genetics Cell Differentiation* Coculture Techniques Collagen / metabolism* Extracellular Matrix / metabolism* Gene Expression / drug effects Male Membrane Glycoproteins / biosynthesis* Membrane Glycoproteins / genetics Mice Osteoclasts / cytology* Osteoclasts / metabolism RANK Ligand RNA, Messenger / analysis RNA, Messenger / metabolism Receptor Activator of Nuclear Factor-kappa B Reverse Transcriptase Polymerase Chain Reaction Stromal Cells |
| IF | 3.934 |
| 引用数 | 32 |
| WOS 分野 | ENDOCRINOLOGY & METABOLISM |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | ST2(RCB0224) |