RRC ID 39526
Author Yang XL, Matsuura H, Fu Y, Sugiyama T, Miura N.
Title MFH-1 is required for bone morphogenetic protein-2-induced osteoblastic differentiation of C2C12 myoblasts.
Journal FEBS Lett
Abstract Mesenchyme forkhead-1 (MFH-1), a winged helix/forkhead transcription factor, is expressed in developing cartilaginous tissues, kidney and arch arteries, and is essential for the normal development of the axial skeleton and aortic arch formation of mice. To investigate the possible role of MFH-1 in osteogenesis and osteoblast differentiation, we examined expression of MFH-1 induced by bone morphogenetic protein-2 (BMP-2) in C2C12 myoblasts, and found that MFH-1 protein and also MFH-1 mRNA increased markedly in C2C12 cells after treatment with BMP-2. To confirm the hypothesis that BMP-2 induced osteoblastic differentiation of C2C12 cells by increasing MFH-1 expression, we lowered the endogenous MFH-1 level by stably transfecting C2C12 cells with antisense MFH-1 sequence, and found that in antisense MFH-1 cell lines, both alkaline phosphatase (ALP) activity and production of osteocalcin induced by BMP-2 decreased markedly in comparison with control cell lines. Our results suggest that the BMP-2-induced MFH-1 protein may play a key role in regulating the commitment to osteoblastic differentiation of C2C12 myoblasts and production of osteoblast markers including ALP and osteocalcin.
Volume 470(1)
Pages 29-34
Published 2000-3-17
DOI 10.1016/s0014-5793(00)01285-0
PII S0014-5793(00)01285-0
PMID 10722840
MeSH Animals Antibodies, Monoclonal / metabolism Antibody Specificity Bone Morphogenetic Protein 2 Bone Morphogenetic Proteins / metabolism* Bone Morphogenetic Proteins / pharmacology Cell Differentiation / drug effects Cell Line DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Forkhead Transcription Factors Gene Expression L Cells Mice Oligonucleotides, Antisense Osteoblasts / cytology* Osteoblasts / drug effects RNA, Messenger Rats Rats, Wistar Transcription Factors / genetics Transcription Factors / metabolism* Transfection Transforming Growth Factor beta*
IF 3.057
Times Cited 19
Human and Animal Cells C2C12(RCB0987)