Reference - Detail
| RRC ID | 39529 |
|---|---|
| Author | Ogata S, Okumura K, Taguchi H. |
| Title | A simple and rapid method for the detection of poly(ADP-ribose) by flow cytometry. |
| Journal | Biosci Biotechnol Biochem |
| Abstract |
Measurement of poly(ADP-ribose) levels was performed by a new method using a monoclonal antibody against poly(ADP-ribose) and flow cytometry from small amount of cultured cells without the need for isolation of poly(ADP-ribose) polymer. The increase of poly(ADP-ribose)-associated fluorescence intensity was observed in individual human leukemic HL-60 cells when treated with the carcinogen, N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), and was blocked by the treatment with 3-aminobenzamide before MNNG treatment. It is easy and rapid to detect the time-dependent change of poly(ADP-ribose) levels in HL-60 cells after MNNG treatment. We easily found that the increase of the poly(ADP-ribose) level in nicotinic acid-treated lymphocytes after MNNG treatment was observed, but not in nicotinamide-treated lymphocytes. We investigated the change of poly(ADP-ribose) levels especially in the early phase of apoptosis. Our method is simple and rapid. It is suggested that the investigation of poly(ADP-ribosyl)ation in various fields is possible by using this new method. |
| Volume | 64(3) |
| Pages | 510-5 |
| Published | 2000-3-1 |
| DOI | 10.1271/bbb.64.510 |
| PMID | 10803947 |
| MeSH | Benzamides / pharmacology Enzyme Inhibitors / pharmacology Flow Cytometry / methods* HL-60 Cells Humans Methylnitronitrosoguanidine / pharmacology Poly Adenosine Diphosphate Ribose / analysis* Poly(ADP-ribose) Polymerase Inhibitors |
| IF | 1.516 |
| Times Cited | 7 |
| WOS Category | CHEMISTRY, APPLIED FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| Human and Animal Cells | |